Abstract
Triglyceride turnover in the isolated C57/BL6 mouse heart was measured by dynamic 13C edit-1H observe NMR and the rate of fatty acid oxidation was determined by 13C NMR isotopomer analysis. In the presence of a physiological mixture of substrates, energy was produced in the citric acid cycle by oxidation of long-chain fatty acids (18%), ketones (34%), lactate (24%), pyruvate (7%), and other sources (17%). Exogenous fatty acids appeared in the triglyceride pool at 0.24 μmol/g dry wt/min, similar to the rate of oxidation of long-chain fatty acids, 0.16 μmol/g dry wt/min. Isoproterenol decreased the rate of de novo triglyceride synthesis and increased the rate of fatty acid oxidation.
Original language | English (US) |
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Pages (from-to) | 4282-4287 |
Number of pages | 6 |
Journal | FEBS Letters |
Volume | 580 |
Issue number | 17 |
DOIs | |
State | Published - Jul 24 2006 |
Keywords
- Carbon-13
- Citric acid cycle
- Fatty acids
- Heart metabolism
- Isotopomer analysis
- Triglycerides
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology