Storage and oxidation of long-chain fatty acids in the C57/BL6 mouse heart as measured by NMR spectroscopy

Kimberly A. Stowe, Shawn C. Burgess, Matthew Merritt, A. Dean Sherry, Craig R. Malloy

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Triglyceride turnover in the isolated C57/BL6 mouse heart was measured by dynamic 13C edit-1H observe NMR and the rate of fatty acid oxidation was determined by 13C NMR isotopomer analysis. In the presence of a physiological mixture of substrates, energy was produced in the citric acid cycle by oxidation of long-chain fatty acids (18%), ketones (34%), lactate (24%), pyruvate (7%), and other sources (17%). Exogenous fatty acids appeared in the triglyceride pool at 0.24 μmol/g dry wt/min, similar to the rate of oxidation of long-chain fatty acids, 0.16 μmol/g dry wt/min. Isoproterenol decreased the rate of de novo triglyceride synthesis and increased the rate of fatty acid oxidation.

Original languageEnglish (US)
Pages (from-to)4282-4287
Number of pages6
JournalFEBS Letters
Volume580
Issue number17
DOIs
StatePublished - Jul 24 2006

Keywords

  • Carbon-13
  • Citric acid cycle
  • Fatty acids
  • Heart metabolism
  • Isotopomer analysis
  • Triglycerides

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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