Strand-specific postreplicative processing of mammalian telomeres

S. M. Bailey, M. N. Cornforth, A. Kurimasa, D. J. Chen, E. H. Goodwin

Research output: Contribution to journalArticle

206 Citations (Scopus)

Abstract

Telomeres are specialized nucleoprotein structures that stabilize the ends of linear eukaryotic chromosomes. In mammalian cells, abrogation of telomeric repeat binding factor TRF2 or DNA-dependent protein kinase (DNA-PK) activity causes end-to-end chromosomal fusion, thus establishing an essential role for these proteins in telomere function. Here we show that TRF2-mediated end-capping occurs after telomere replication. The postreplicative requirement for TRF2 and DNA-PKcs, the catalytic subunit of DNA-PK, is confined to only haft of the telomeres, namely, those that were produced by leading-strand DNA synthesis. These results demonstrate a crucial difference in postreplicative processing of telomeres that is linked to their mode of replication.

Original languageEnglish (US)
Pages (from-to)2462-2465
Number of pages4
JournalScience
Volume293
Issue number5539
DOIs
StatePublished - Sep 28 2001

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Telomere
DNA-Activated Protein Kinase
Catalytic DNA
Nucleoproteins
DNA
Catalytic Domain
Chromosomes
Proteins

ASJC Scopus subject areas

  • General

Cite this

Bailey, S. M., Cornforth, M. N., Kurimasa, A., Chen, D. J., & Goodwin, E. H. (2001). Strand-specific postreplicative processing of mammalian telomeres. Science, 293(5539), 2462-2465. https://doi.org/10.1126/science.1062560

Strand-specific postreplicative processing of mammalian telomeres. / Bailey, S. M.; Cornforth, M. N.; Kurimasa, A.; Chen, D. J.; Goodwin, E. H.

In: Science, Vol. 293, No. 5539, 28.09.2001, p. 2462-2465.

Research output: Contribution to journalArticle

Bailey, SM, Cornforth, MN, Kurimasa, A, Chen, DJ & Goodwin, EH 2001, 'Strand-specific postreplicative processing of mammalian telomeres', Science, vol. 293, no. 5539, pp. 2462-2465. https://doi.org/10.1126/science.1062560
Bailey SM, Cornforth MN, Kurimasa A, Chen DJ, Goodwin EH. Strand-specific postreplicative processing of mammalian telomeres. Science. 2001 Sep 28;293(5539):2462-2465. https://doi.org/10.1126/science.1062560
Bailey, S. M. ; Cornforth, M. N. ; Kurimasa, A. ; Chen, D. J. ; Goodwin, E. H. / Strand-specific postreplicative processing of mammalian telomeres. In: Science. 2001 ; Vol. 293, No. 5539. pp. 2462-2465.
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