Strategies for top-down proteomics of Methanococcus jannaschii and Saccharomyces cerevisiae using a quadrupole FT-ICR-MS

Steven M. Patrie, Jay Charlebois, Derek Vander Molen, Fanyu Meng, Andrew Forbes, John P. Quinn, Christopher L. Hendrickson, Alan G. Marshall, Neil L. Kelleher

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The Proteomics of methanococcus jannaschii and saccharomyces cerevisiae were studied using a quadrupole FT-ICR-MS. It was found that the top down approach fragments intact proteins within the mass spectrometer, which allowed more sequence coverage. It was shown that the micro-electrosprayed ion was desolvated and transported for accumulation either by placing the quadrupole in rf-only or mass filtered by quadrupole. Analysis shows that post transcription processing events increased proteome complexity which ultimately increased the database complexity for larger proteins in higher order organisms.

Original languageEnglish (US)
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages687-688
Number of pages2
StatePublished - 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

ASJC Scopus subject areas

  • Spectroscopy

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    Patrie, S. M., Charlebois, J., Vander Molen, D., Meng, F., Forbes, A., Quinn, J. P., Hendrickson, C. L., Marshall, A. G., & Kelleher, N. L. (2002). Strategies for top-down proteomics of Methanococcus jannaschii and Saccharomyces cerevisiae using a quadrupole FT-ICR-MS. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 687-688)