Stromal CD10 and SPARC expression in ductal carcinoma in situ (DCIS) patients predicts disease recurrence

Agnieszka K. Witkiewicz, Boris Freydin, Inna Chervoneva, Magdalena Potoczek, Wendy Rizzo, Hallgeir Rui, Jonathan R. Brody, Gordon F. Schwartz, Michael P. Lisanti

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

The current classification of ductal carcinoma in situ (DCIS) is based on nuclear grade, architectural differentiation and the presence of necrosis that does not adequately predict the likelihood of recurrence after breast conserving therapy; therefore, there is a critical need to identify novel predictors of DCIS progression. Ninety seven cases of DCIS were included in the study. CD10 and SP ARC expression in tumor stroma was assessed by standard immunoperoxidase method with ani-CD 10 and anti-SP ARC antibodies. The staining was scored semi-quantitatively as negative, weak or strong. Statistical analysis was performed using the Fisher's exact test. Multivariable analysis was conducted utilizing Exact Logistic Regression software (SAS 9.1 and LogExact). A significant association was observed between the recurrence status and time to recurrence with expression of CD10 (p < 0.001) and SP ARC (p < 0.001). When combining both SP ARC and CD10 expression there was a strong correlation with the shortest time to recurrence. Stromal CD10 and SP ARC expression are new markers of an increased risk for DCIS recurrence, independent of commonly assessed clinical parameters. Thus, stromal CD10 and SP ARC expression levels are promising markers of DCIS recurrence and warrant evaluation in larger prospective studies.

Original languageEnglish (US)
Pages (from-to)391-396
Number of pages6
JournalCancer Biology and Therapy
Volume10
Issue number4
DOIs
StatePublished - Aug 15 2010

    Fingerprint

Keywords

  • CD10
  • Cancer stroma
  • DCIS
  • SPARC

ASJC Scopus subject areas

  • Molecular Medicine
  • Oncology
  • Pharmacology
  • Cancer Research

Cite this

Witkiewicz, A. K., Freydin, B., Chervoneva, I., Potoczek, M., Rizzo, W., Rui, H., Brody, J. R., Schwartz, G. F., & Lisanti, M. P. (2010). Stromal CD10 and SPARC expression in ductal carcinoma in situ (DCIS) patients predicts disease recurrence. Cancer Biology and Therapy, 10(4), 391-396. https://doi.org/10.4161/cbt.10.4.12449