Stromal Hedgehog pathway activation by IHH suppresses lung adenocarcinoma growth and metastasis by limiting reactive oxygen species

Sahba Kasiri, Baozhi Chen, Alexandra N. Wilson, Annika Reczek, Simbarashe Mazambani, Jashkaran Gadhvi, Evan Noel, Ummay Marriam, Barbara Mino, Wei Lu, Luc Girard, Luisa M. Solis, Katherine Luby-Phelps, Justin Bishop, Jung Whan Kim, James Kim

Research output: Contribution to journalArticle

Abstract

Activation of the Hedgehog (Hh) signaling pathway by mutations within its components drives the growth of several cancers. However, the role of Hh pathway activation in lung cancers has been controversial. Here, we demonstrate that the canonical Hh signaling pathway is activated in lung stroma by Hh ligands secreted from transformed lung epithelia. Genetic deletion of Shh, the primary Hh ligand expressed in the lung, in KrasG12D/+;Trp53fl/fl autochthonous murine lung adenocarcinoma had no effect on survival. Early abrogation of the pathway by an anti-SHH/IHH antibody 5E1 led to significantly worse survival with increased tumor and metastatic burden. Loss of IHH, another Hh ligand, by in vivo CRISPR led to more aggressive tumor growth suggesting that IHH, rather than SHH, activates the pathway in stroma to drive its tumor suppressive effects—a novel role for IHH in the lung. Tumors from mice treated with 5E1 had decreased blood vessel density and increased DNA damage suggestive of reactive oxygen species (ROS) activity. Treatment of KrasG12D/+;Trp53fl/fl mice with 5E1 and N-acetylcysteine, as a ROS scavenger, decreased tumor DNA damage, inhibited tumor growth and prolonged mouse survival. Thus, IHH induces stromal activation of the canonical Hh signaling pathway to suppress tumor growth and metastases, in part, by limiting ROS activity.

Original languageEnglish (US)
Pages (from-to)3258-3275
Number of pages18
JournalOncogene
Volume39
Issue number16
DOIs
StatePublished - Apr 16 2020

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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  • Cite this

    Kasiri, S., Chen, B., Wilson, A. N., Reczek, A., Mazambani, S., Gadhvi, J., Noel, E., Marriam, U., Mino, B., Lu, W., Girard, L., Solis, L. M., Luby-Phelps, K., Bishop, J., Kim, J. W., & Kim, J. (2020). Stromal Hedgehog pathway activation by IHH suppresses lung adenocarcinoma growth and metastasis by limiting reactive oxygen species. Oncogene, 39(16), 3258-3275. https://doi.org/10.1038/s41388-020-1224-5