Structural analysis of a human I-A homologue using a monoclonal antibody that recognizes an MB3-like specificity

R. C. Giles, G. Nunez, C. K. Hurley, A. Nunez-Roldan, R. Winchester, P. Stastny, J. D. Capra

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Monoclonal antibody IVD12 was used to isolate and characterize a human Ia molecule present on B cells that generally display DR4 or DR5 phenotypes. The specificity of binding of IVD12 to human peripheral blood B cells from 75 normal individuals and 19 homozygous human lymphoblastoid B cell lines was identical to the supertypic specificity MB3 previously defined. Furthermore, IVD12-reactivity was shown to segregate with HLA in three informative families. In each family, individuals positive for IVD12 binding were also positive for DR4 or DR5. Using IVD12, a molecule has been isolated from the homozygous cell line PRIESS (DR4/4) and has been shown by amino acid sequence analysis to be homologous to the murine I-A and human HLA-DS molecules. These findings suggest that the MB3 specificity is found on a molecule encoded by loci distinct from those loci which encode HLA-DR molecules. This molecule represents the third family of HLA-D region molecules isolated from the cell line PRIESS. Both HLA-DR and HLA-SB molecules from this cell line were previously shown by amino acid sequence analysis to be I-E-like but distinct from one another. Collectively, these data provide evidence that the HLA-D region contains at least six loci encoding distinct alpha and beta chains for the HLA-SB, HLA-DR, and HLA-DS molecules.

Original languageEnglish (US)
Pages (from-to)1461-1470
Number of pages10
JournalJournal of Experimental Medicine
Volume157
Issue number5
StatePublished - 1983

Fingerprint

HLA-DR Antigens
HLA-DP Antigens
Monoclonal Antibodies
HLA-D Antigens
HLA-DQ Antigens
Cell Line
B-Lymphocytes
Protein Sequence Analysis
Blood Cells
Phenotype

ASJC Scopus subject areas

  • Immunology

Cite this

Giles, R. C., Nunez, G., Hurley, C. K., Nunez-Roldan, A., Winchester, R., Stastny, P., & Capra, J. D. (1983). Structural analysis of a human I-A homologue using a monoclonal antibody that recognizes an MB3-like specificity. Journal of Experimental Medicine, 157(5), 1461-1470.

Structural analysis of a human I-A homologue using a monoclonal antibody that recognizes an MB3-like specificity. / Giles, R. C.; Nunez, G.; Hurley, C. K.; Nunez-Roldan, A.; Winchester, R.; Stastny, P.; Capra, J. D.

In: Journal of Experimental Medicine, Vol. 157, No. 5, 1983, p. 1461-1470.

Research output: Contribution to journalArticle

Giles, RC, Nunez, G, Hurley, CK, Nunez-Roldan, A, Winchester, R, Stastny, P & Capra, JD 1983, 'Structural analysis of a human I-A homologue using a monoclonal antibody that recognizes an MB3-like specificity', Journal of Experimental Medicine, vol. 157, no. 5, pp. 1461-1470.
Giles RC, Nunez G, Hurley CK, Nunez-Roldan A, Winchester R, Stastny P et al. Structural analysis of a human I-A homologue using a monoclonal antibody that recognizes an MB3-like specificity. Journal of Experimental Medicine. 1983;157(5):1461-1470.
Giles, R. C. ; Nunez, G. ; Hurley, C. K. ; Nunez-Roldan, A. ; Winchester, R. ; Stastny, P. ; Capra, J. D. / Structural analysis of a human I-A homologue using a monoclonal antibody that recognizes an MB3-like specificity. In: Journal of Experimental Medicine. 1983 ; Vol. 157, No. 5. pp. 1461-1470.
@article{5ad95d3627bb4eccadcd0daf8c93257b,
title = "Structural analysis of a human I-A homologue using a monoclonal antibody that recognizes an MB3-like specificity",
abstract = "Monoclonal antibody IVD12 was used to isolate and characterize a human Ia molecule present on B cells that generally display DR4 or DR5 phenotypes. The specificity of binding of IVD12 to human peripheral blood B cells from 75 normal individuals and 19 homozygous human lymphoblastoid B cell lines was identical to the supertypic specificity MB3 previously defined. Furthermore, IVD12-reactivity was shown to segregate with HLA in three informative families. In each family, individuals positive for IVD12 binding were also positive for DR4 or DR5. Using IVD12, a molecule has been isolated from the homozygous cell line PRIESS (DR4/4) and has been shown by amino acid sequence analysis to be homologous to the murine I-A and human HLA-DS molecules. These findings suggest that the MB3 specificity is found on a molecule encoded by loci distinct from those loci which encode HLA-DR molecules. This molecule represents the third family of HLA-D region molecules isolated from the cell line PRIESS. Both HLA-DR and HLA-SB molecules from this cell line were previously shown by amino acid sequence analysis to be I-E-like but distinct from one another. Collectively, these data provide evidence that the HLA-D region contains at least six loci encoding distinct alpha and beta chains for the HLA-SB, HLA-DR, and HLA-DS molecules.",
author = "Giles, {R. C.} and G. Nunez and Hurley, {C. K.} and A. Nunez-Roldan and R. Winchester and P. Stastny and Capra, {J. D.}",
year = "1983",
language = "English (US)",
volume = "157",
pages = "1461--1470",
journal = "Journal of Experimental Medicine",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "5",

}

TY - JOUR

T1 - Structural analysis of a human I-A homologue using a monoclonal antibody that recognizes an MB3-like specificity

AU - Giles, R. C.

AU - Nunez, G.

AU - Hurley, C. K.

AU - Nunez-Roldan, A.

AU - Winchester, R.

AU - Stastny, P.

AU - Capra, J. D.

PY - 1983

Y1 - 1983

N2 - Monoclonal antibody IVD12 was used to isolate and characterize a human Ia molecule present on B cells that generally display DR4 or DR5 phenotypes. The specificity of binding of IVD12 to human peripheral blood B cells from 75 normal individuals and 19 homozygous human lymphoblastoid B cell lines was identical to the supertypic specificity MB3 previously defined. Furthermore, IVD12-reactivity was shown to segregate with HLA in three informative families. In each family, individuals positive for IVD12 binding were also positive for DR4 or DR5. Using IVD12, a molecule has been isolated from the homozygous cell line PRIESS (DR4/4) and has been shown by amino acid sequence analysis to be homologous to the murine I-A and human HLA-DS molecules. These findings suggest that the MB3 specificity is found on a molecule encoded by loci distinct from those loci which encode HLA-DR molecules. This molecule represents the third family of HLA-D region molecules isolated from the cell line PRIESS. Both HLA-DR and HLA-SB molecules from this cell line were previously shown by amino acid sequence analysis to be I-E-like but distinct from one another. Collectively, these data provide evidence that the HLA-D region contains at least six loci encoding distinct alpha and beta chains for the HLA-SB, HLA-DR, and HLA-DS molecules.

AB - Monoclonal antibody IVD12 was used to isolate and characterize a human Ia molecule present on B cells that generally display DR4 or DR5 phenotypes. The specificity of binding of IVD12 to human peripheral blood B cells from 75 normal individuals and 19 homozygous human lymphoblastoid B cell lines was identical to the supertypic specificity MB3 previously defined. Furthermore, IVD12-reactivity was shown to segregate with HLA in three informative families. In each family, individuals positive for IVD12 binding were also positive for DR4 or DR5. Using IVD12, a molecule has been isolated from the homozygous cell line PRIESS (DR4/4) and has been shown by amino acid sequence analysis to be homologous to the murine I-A and human HLA-DS molecules. These findings suggest that the MB3 specificity is found on a molecule encoded by loci distinct from those loci which encode HLA-DR molecules. This molecule represents the third family of HLA-D region molecules isolated from the cell line PRIESS. Both HLA-DR and HLA-SB molecules from this cell line were previously shown by amino acid sequence analysis to be I-E-like but distinct from one another. Collectively, these data provide evidence that the HLA-D region contains at least six loci encoding distinct alpha and beta chains for the HLA-SB, HLA-DR, and HLA-DS molecules.

UR - http://www.scopus.com/inward/record.url?scp=0020567418&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020567418&partnerID=8YFLogxK

M3 - Article

C2 - 6189938

AN - SCOPUS:0020567418

VL - 157

SP - 1461

EP - 1470

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

SN - 0022-1007

IS - 5

ER -