Structural analysis of conserved oligomeric Golgi complex subunit 2

Lorraine F. Cavanaugh, Xiaocheng Chen, Brian C. Richardson, Daniel Ungar, Istvan Pelczer, Jose Rizo-Rey, Frederick M. Hughson

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The conserved oligomeric Golgi (COG) complex is strongly implicated in retrograde vesicular trafficking within the Golgi apparatus. Although its mechanism of action is poorly understood, it has been proposed to function by mediating the initial physical contact between transport vesicles and their membrane targets. An analogous role in tethering vesicles has been suggested for at least six additional large multisubunit complexes, including the exocyst, a complex essential for trafficking to the plasma membrane. Here we report the solution structure of a large portion of yeast Cog2p, one of eight subunits composing the COG complex. The structure reveals a six-helix bundle with few conserved surface features but a general resemblance to recently determined crystal structures of four different exocyst subunits. This finding provides the first structural evidence that COG, like the exocyst and potentially other tethering complexes, is constructed from helical bundles. These structures may represent platforms for interaction with other trafficking proteins including SNAREs (soluble N-ethylmaleimide factor attachment protein receptors) and Rabs.

Original languageEnglish (US)
Pages (from-to)23418-23426
Number of pages9
JournalJournal of Biological Chemistry
Volume282
Issue number32
DOIs
StatePublished - Aug 10 2007

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Golgi Apparatus
Structural analysis
Ethylmaleimide
Cell membranes
Yeast
Transport Vesicles
Proteins
Crystal structure
Protein Transport
Membranes
Yeasts
Cell Membrane

ASJC Scopus subject areas

  • Biochemistry

Cite this

Cavanaugh, L. F., Chen, X., Richardson, B. C., Ungar, D., Pelczer, I., Rizo-Rey, J., & Hughson, F. M. (2007). Structural analysis of conserved oligomeric Golgi complex subunit 2. Journal of Biological Chemistry, 282(32), 23418-23426. https://doi.org/10.1074/jbc.M703716200

Structural analysis of conserved oligomeric Golgi complex subunit 2. / Cavanaugh, Lorraine F.; Chen, Xiaocheng; Richardson, Brian C.; Ungar, Daniel; Pelczer, Istvan; Rizo-Rey, Jose; Hughson, Frederick M.

In: Journal of Biological Chemistry, Vol. 282, No. 32, 10.08.2007, p. 23418-23426.

Research output: Contribution to journalArticle

Cavanaugh, LF, Chen, X, Richardson, BC, Ungar, D, Pelczer, I, Rizo-Rey, J & Hughson, FM 2007, 'Structural analysis of conserved oligomeric Golgi complex subunit 2', Journal of Biological Chemistry, vol. 282, no. 32, pp. 23418-23426. https://doi.org/10.1074/jbc.M703716200
Cavanaugh LF, Chen X, Richardson BC, Ungar D, Pelczer I, Rizo-Rey J et al. Structural analysis of conserved oligomeric Golgi complex subunit 2. Journal of Biological Chemistry. 2007 Aug 10;282(32):23418-23426. https://doi.org/10.1074/jbc.M703716200
Cavanaugh, Lorraine F. ; Chen, Xiaocheng ; Richardson, Brian C. ; Ungar, Daniel ; Pelczer, Istvan ; Rizo-Rey, Jose ; Hughson, Frederick M. / Structural analysis of conserved oligomeric Golgi complex subunit 2. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 32. pp. 23418-23426.
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