Structural analysis of the gene encoding human aromatase cytochrome P-450, the enzyme responsible for estrogen biosynthesis

G. D. Means, M. S. Mahendroo, C. J. Corbin, J. M. Mathis, F. E. Powell, C. R. Mendelson, E. R. Simpson

Research output: Contribution to journalArticle

307 Citations (Scopus)

Abstract

The structural gene encoding aromatase cytochrome P-450 (P-450(AROM)) was isolated from human genomic DNA. The gene spans at least 52 kilobases and is composed of 10 exons, the first of which is untranslated. Analysis of the transcription initiation site of human P-450(AROM) mRNA reveals the differential use of 1 of 3 consecutive G residues at the cap site. DNA sequence analysis indicates that the gene has a putative TATA (ATAAAA) sequence at -23 base pairs (bp) and putative CAAT binding sequences beginning at -41, -67, and -83 bp. The 5'-flanking region contains sequences similar to consensus sequences of cis-acting elements defined as regulators of aromatase gene expression. These putative sequences include a cAMP regulatory element at -211 bp, an AP1 (protein kinase C) site at -54 bp, and glucocorticoid regulatory elements at -352 bp and within the first intron at +346 bp. There appears to be only one gene encoding P-450(AROM) in the human genome. Two major species of human P-450(AROM) mRNA (3.4 and 2.9 kilobases) are derived from the use of two polyadenylation signals.

Original languageEnglish (US)
Pages (from-to)19385-19391
Number of pages7
JournalJournal of Biological Chemistry
Volume264
Issue number32
StatePublished - 1989

Fingerprint

Gene encoding
Aromatase
Biosynthesis
Structural analysis
Base Pairing
Cytochrome P-450 Enzyme System
Estrogens
Genes
Messenger RNA
5' Flanking Region
Transcription Initiation Site
DNA sequences
Gene expression
Introns
Protein Kinase C
Glucocorticoids
Exons
Polyadenylation
DNA
Consensus Sequence

ASJC Scopus subject areas

  • Biochemistry

Cite this

Structural analysis of the gene encoding human aromatase cytochrome P-450, the enzyme responsible for estrogen biosynthesis. / Means, G. D.; Mahendroo, M. S.; Corbin, C. J.; Mathis, J. M.; Powell, F. E.; Mendelson, C. R.; Simpson, E. R.

In: Journal of Biological Chemistry, Vol. 264, No. 32, 1989, p. 19385-19391.

Research output: Contribution to journalArticle

Means, G. D. ; Mahendroo, M. S. ; Corbin, C. J. ; Mathis, J. M. ; Powell, F. E. ; Mendelson, C. R. ; Simpson, E. R. / Structural analysis of the gene encoding human aromatase cytochrome P-450, the enzyme responsible for estrogen biosynthesis. In: Journal of Biological Chemistry. 1989 ; Vol. 264, No. 32. pp. 19385-19391.
@article{b40c9124d8b249608265245cc52601fb,
title = "Structural analysis of the gene encoding human aromatase cytochrome P-450, the enzyme responsible for estrogen biosynthesis",
abstract = "The structural gene encoding aromatase cytochrome P-450 (P-450(AROM)) was isolated from human genomic DNA. The gene spans at least 52 kilobases and is composed of 10 exons, the first of which is untranslated. Analysis of the transcription initiation site of human P-450(AROM) mRNA reveals the differential use of 1 of 3 consecutive G residues at the cap site. DNA sequence analysis indicates that the gene has a putative TATA (ATAAAA) sequence at -23 base pairs (bp) and putative CAAT binding sequences beginning at -41, -67, and -83 bp. The 5'-flanking region contains sequences similar to consensus sequences of cis-acting elements defined as regulators of aromatase gene expression. These putative sequences include a cAMP regulatory element at -211 bp, an AP1 (protein kinase C) site at -54 bp, and glucocorticoid regulatory elements at -352 bp and within the first intron at +346 bp. There appears to be only one gene encoding P-450(AROM) in the human genome. Two major species of human P-450(AROM) mRNA (3.4 and 2.9 kilobases) are derived from the use of two polyadenylation signals.",
author = "Means, {G. D.} and Mahendroo, {M. S.} and Corbin, {C. J.} and Mathis, {J. M.} and Powell, {F. E.} and Mendelson, {C. R.} and Simpson, {E. R.}",
year = "1989",
language = "English (US)",
volume = "264",
pages = "19385--19391",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "32",

}

TY - JOUR

T1 - Structural analysis of the gene encoding human aromatase cytochrome P-450, the enzyme responsible for estrogen biosynthesis

AU - Means, G. D.

AU - Mahendroo, M. S.

AU - Corbin, C. J.

AU - Mathis, J. M.

AU - Powell, F. E.

AU - Mendelson, C. R.

AU - Simpson, E. R.

PY - 1989

Y1 - 1989

N2 - The structural gene encoding aromatase cytochrome P-450 (P-450(AROM)) was isolated from human genomic DNA. The gene spans at least 52 kilobases and is composed of 10 exons, the first of which is untranslated. Analysis of the transcription initiation site of human P-450(AROM) mRNA reveals the differential use of 1 of 3 consecutive G residues at the cap site. DNA sequence analysis indicates that the gene has a putative TATA (ATAAAA) sequence at -23 base pairs (bp) and putative CAAT binding sequences beginning at -41, -67, and -83 bp. The 5'-flanking region contains sequences similar to consensus sequences of cis-acting elements defined as regulators of aromatase gene expression. These putative sequences include a cAMP regulatory element at -211 bp, an AP1 (protein kinase C) site at -54 bp, and glucocorticoid regulatory elements at -352 bp and within the first intron at +346 bp. There appears to be only one gene encoding P-450(AROM) in the human genome. Two major species of human P-450(AROM) mRNA (3.4 and 2.9 kilobases) are derived from the use of two polyadenylation signals.

AB - The structural gene encoding aromatase cytochrome P-450 (P-450(AROM)) was isolated from human genomic DNA. The gene spans at least 52 kilobases and is composed of 10 exons, the first of which is untranslated. Analysis of the transcription initiation site of human P-450(AROM) mRNA reveals the differential use of 1 of 3 consecutive G residues at the cap site. DNA sequence analysis indicates that the gene has a putative TATA (ATAAAA) sequence at -23 base pairs (bp) and putative CAAT binding sequences beginning at -41, -67, and -83 bp. The 5'-flanking region contains sequences similar to consensus sequences of cis-acting elements defined as regulators of aromatase gene expression. These putative sequences include a cAMP regulatory element at -211 bp, an AP1 (protein kinase C) site at -54 bp, and glucocorticoid regulatory elements at -352 bp and within the first intron at +346 bp. There appears to be only one gene encoding P-450(AROM) in the human genome. Two major species of human P-450(AROM) mRNA (3.4 and 2.9 kilobases) are derived from the use of two polyadenylation signals.

UR - http://www.scopus.com/inward/record.url?scp=0024853552&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024853552&partnerID=8YFLogxK

M3 - Article

C2 - 2808431

AN - SCOPUS:0024853552

VL - 264

SP - 19385

EP - 19391

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 32

ER -