Structural and genetic studies on chicken 7S immunoglobulin allotypes. II. Distribution of allotypes on the 7S immunoglobulin of homozygous and heterozygous chickens

E. K. Wakeland, A. A. Benedict, H. A. Abplanalp

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Abstract

We have previously reported that chicken 7S immunoglobulin (Ig) heavy (H) chain allotypes (CS 1 locus) segregate as phenogroups in F2 progeny. Specificity CS 1.1 formed a phenogroup with CS 1.4 in inbred chicken line UCD 2, and a second phenogroup with CS 1.3 in line UCD 3. To determine whether these phenogroups were formed by combinations of specificities on the same, or on separate subclasses of 7S Ig, their distribution on the 7S Ig molecules of birds homozygous for 7S Ig allotypes was analyzed by radioimmunoassay. Anti CS 1.1 and anti CS 1.3 alloantisera each bound more than 94% of line UCD 3 125I 7S Ig. Similar results were obtained with alloantisera to CS 1.1 and CS 1.4 with 125I 7S Ig from line UCD 2. These results indicate that both phenogroups were formed by combinations of specificities present on the same H chain. Thus, each phenogroup described, probably, is the product of a single structural gene which is responsible for more than 94% of the 7S Ig H chain constant regions. In F1 hybrids with the genotype CS 1.1, 1.3/CS 1.2, two populations of serum 7S Ig molecules were detected by direct and sequential binding analysis with specific alloantisera. One population of 7S Ig contained specificities CS 1.1 and CS 1.3, but not CS 1.2; while the second population carried only specificity CS 1.2. Therefore, each population was exclusively the product of one parental allele. Consistent with a genetic regulatory mechanism involving allelic exclusion, no 7S Ig containing allotypes produced by both alleles was detected.

Original languageEnglish (US)
Pages (from-to)401-404
Number of pages4
JournalJournal of Immunology
Volume118
Issue number2
StatePublished - 1977

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Immunoglobulin Allotypes
Immunoglobulins
Chickens
Population
Immunoglobulin Subunits
Alleles
Immunoglobulin Heavy Chains
Birds
Radioimmunoassay
Genotype
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

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title = "Structural and genetic studies on chicken 7S immunoglobulin allotypes. II. Distribution of allotypes on the 7S immunoglobulin of homozygous and heterozygous chickens",
abstract = "We have previously reported that chicken 7S immunoglobulin (Ig) heavy (H) chain allotypes (CS 1 locus) segregate as phenogroups in F2 progeny. Specificity CS 1.1 formed a phenogroup with CS 1.4 in inbred chicken line UCD 2, and a second phenogroup with CS 1.3 in line UCD 3. To determine whether these phenogroups were formed by combinations of specificities on the same, or on separate subclasses of 7S Ig, their distribution on the 7S Ig molecules of birds homozygous for 7S Ig allotypes was analyzed by radioimmunoassay. Anti CS 1.1 and anti CS 1.3 alloantisera each bound more than 94{\%} of line UCD 3 125I 7S Ig. Similar results were obtained with alloantisera to CS 1.1 and CS 1.4 with 125I 7S Ig from line UCD 2. These results indicate that both phenogroups were formed by combinations of specificities present on the same H chain. Thus, each phenogroup described, probably, is the product of a single structural gene which is responsible for more than 94{\%} of the 7S Ig H chain constant regions. In F1 hybrids with the genotype CS 1.1, 1.3/CS 1.2, two populations of serum 7S Ig molecules were detected by direct and sequential binding analysis with specific alloantisera. One population of 7S Ig contained specificities CS 1.1 and CS 1.3, but not CS 1.2; while the second population carried only specificity CS 1.2. Therefore, each population was exclusively the product of one parental allele. Consistent with a genetic regulatory mechanism involving allelic exclusion, no 7S Ig containing allotypes produced by both alleles was detected.",
author = "Wakeland, {E. K.} and Benedict, {A. A.} and Abplanalp, {H. A.}",
year = "1977",
language = "English (US)",
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T1 - Structural and genetic studies on chicken 7S immunoglobulin allotypes. II. Distribution of allotypes on the 7S immunoglobulin of homozygous and heterozygous chickens

AU - Wakeland, E. K.

AU - Benedict, A. A.

AU - Abplanalp, H. A.

PY - 1977

Y1 - 1977

N2 - We have previously reported that chicken 7S immunoglobulin (Ig) heavy (H) chain allotypes (CS 1 locus) segregate as phenogroups in F2 progeny. Specificity CS 1.1 formed a phenogroup with CS 1.4 in inbred chicken line UCD 2, and a second phenogroup with CS 1.3 in line UCD 3. To determine whether these phenogroups were formed by combinations of specificities on the same, or on separate subclasses of 7S Ig, their distribution on the 7S Ig molecules of birds homozygous for 7S Ig allotypes was analyzed by radioimmunoassay. Anti CS 1.1 and anti CS 1.3 alloantisera each bound more than 94% of line UCD 3 125I 7S Ig. Similar results were obtained with alloantisera to CS 1.1 and CS 1.4 with 125I 7S Ig from line UCD 2. These results indicate that both phenogroups were formed by combinations of specificities present on the same H chain. Thus, each phenogroup described, probably, is the product of a single structural gene which is responsible for more than 94% of the 7S Ig H chain constant regions. In F1 hybrids with the genotype CS 1.1, 1.3/CS 1.2, two populations of serum 7S Ig molecules were detected by direct and sequential binding analysis with specific alloantisera. One population of 7S Ig contained specificities CS 1.1 and CS 1.3, but not CS 1.2; while the second population carried only specificity CS 1.2. Therefore, each population was exclusively the product of one parental allele. Consistent with a genetic regulatory mechanism involving allelic exclusion, no 7S Ig containing allotypes produced by both alleles was detected.

AB - We have previously reported that chicken 7S immunoglobulin (Ig) heavy (H) chain allotypes (CS 1 locus) segregate as phenogroups in F2 progeny. Specificity CS 1.1 formed a phenogroup with CS 1.4 in inbred chicken line UCD 2, and a second phenogroup with CS 1.3 in line UCD 3. To determine whether these phenogroups were formed by combinations of specificities on the same, or on separate subclasses of 7S Ig, their distribution on the 7S Ig molecules of birds homozygous for 7S Ig allotypes was analyzed by radioimmunoassay. Anti CS 1.1 and anti CS 1.3 alloantisera each bound more than 94% of line UCD 3 125I 7S Ig. Similar results were obtained with alloantisera to CS 1.1 and CS 1.4 with 125I 7S Ig from line UCD 2. These results indicate that both phenogroups were formed by combinations of specificities present on the same H chain. Thus, each phenogroup described, probably, is the product of a single structural gene which is responsible for more than 94% of the 7S Ig H chain constant regions. In F1 hybrids with the genotype CS 1.1, 1.3/CS 1.2, two populations of serum 7S Ig molecules were detected by direct and sequential binding analysis with specific alloantisera. One population of 7S Ig contained specificities CS 1.1 and CS 1.3, but not CS 1.2; while the second population carried only specificity CS 1.2. Therefore, each population was exclusively the product of one parental allele. Consistent with a genetic regulatory mechanism involving allelic exclusion, no 7S Ig containing allotypes produced by both alleles was detected.

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