Structural basis and catalytic mechanism for the dual functional Endo-β-N-acetylglucosaminidase A

Jie Yin, Lei Li, Neil Shaw, Yang Li., Jing Katherine Song, Wenpeng Zhang, Chengfeng Xia, Rongguang Zhang, Andrzej Joachimiak, Hou Cheng Zhang, Lai Xi Wang, Zhi Jie Liu, Peng Wang

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

Endo-β-N-acetylglucosaminidases (ENGases) are dual specificity enzymes with an ability to catalyze hydrolysis and transglycosylation reactions. Recently, these enzymes have become the focus of intense research because of their potential for synthesis of glycopeptides. We have determined the 3D structures of an ENGase from Arthrobacter protophormiae (Endo-A) in 3 forms, one in native form, one in complex with Man3 GlcNAc-thiazoline and another in complex with GlcNAc-Asn. The carbohydrate moiety sits above the TIM-barrel in a cleft region surrounded by aromatic residues. The conserved essential catalytic residues - E173, N171 and Y205 are within hydrogen bonding distance of the substrate. W216 and W244 regulate access to the active site during transglycosylation by serving as "gate-keepers". Interestingly, Y299F mutation resulted in a 3 fold increase in the transglycosylation activity. The structure provides insights into the catalytic mechanism of GH85 family of glycoside hydrolases at molecular level and could assist rational engineering of ENGases.

Original languageEnglish (US)
Article numbere4658
JournalPloS one
Volume4
Issue number3
DOIs
StatePublished - Mar 2 2009
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Fingerprint Dive into the research topics of 'Structural basis and catalytic mechanism for the dual functional Endo-β-N-acetylglucosaminidase A'. Together they form a unique fingerprint.

  • Cite this

    Yin, J., Li, L., Shaw, N., Li., Y., Song, J. K., Zhang, W., Xia, C., Zhang, R., Joachimiak, A., Zhang, H. C., Wang, L. X., Liu, Z. J., & Wang, P. (2009). Structural basis and catalytic mechanism for the dual functional Endo-β-N-acetylglucosaminidase A. PloS one, 4(3), [e4658]. https://doi.org/10.1371/journal.pone.0004658