Structural basis for cooperativity in recruitment of MAML coactivators to Notch transcription complexes

Yunsun Nam, Piotr Sliz, Luyan Song, Jon C. Aster, Stephen C. Blacklow

Research output: Contribution to journalArticle

306 Scopus citations

Abstract

Notch receptors transduce essential developmental signals between neighboring cells by forming a complex that leads to transcription of target genes upon activation. We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). Together, CSL and ANK create a groove to bind the MAML-1 polypeptide as a kinked, 70 Å helix. The composite binding surface likely restricts the recruitment of MAML proteins to promoters on which Notch:CSL complexes have been preassembled, ensuring tight transcriptional control of Notch target genes.

Original languageEnglish (US)
Pages (from-to)973-983
Number of pages11
JournalCell
Volume124
Issue number5
DOIs
StatePublished - Mar 10 2006

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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