Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex

Yadong Sun, Lei Li, Alberto P. Macho, Zhifu Han, Zehan Hu, Cyril Zipfel, Jian Min Zhou, Jijie Chai

Research output: Contribution to journalArticle

294 Citations (Scopus)

Abstract

Flagellin perception in Arabidopsis is through recognition of its highly conserved N-terminal epitope (flg22) by flagellin-sensitive 2 (FLS2). Flg22 binding induces FLS2 heteromerization with BRASSINOSTEROID INSENSITIVE 1-associated kinase 1 (BAK1) and their reciprocal activation followed by plant immunity. Here, we report the crystal structure of FLS2 and BAK1 ectodomains complexed with flg22 at 3.06 angstroms. A conserved and a nonconserved site from the inner surface of the FLS2 solenoid recognize the C- and N-terminal segment of flg22, respectively, without oligomerization or conformational changes in the FLS2 ectodomain. Besides directly interacting with FLS2, BAK1 acts as a co-receptor by recognizing the C terminus of the FLS2-bound flg22. Our data reveal the molecular mechanisms underlying FLS2-BAK1 complex recognition of flg22 and provide insight into the immune receptor complex activation.

Original languageEnglish (US)
Pages (from-to)624-628
Number of pages5
JournalScience
Volume342
Issue number6158
DOIs
StatePublished - Jan 1 2013
Externally publishedYes

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Brassinosteroids
Flagellin
Antigen-Antibody Complex
Arabidopsis
Phosphotransferases
Plant Immunity
Epitopes

ASJC Scopus subject areas

  • General

Cite this

Sun, Y., Li, L., Macho, A. P., Han, Z., Hu, Z., Zipfel, C., ... Chai, J. (2013). Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Science, 342(6158), 624-628. https://doi.org/10.1126/science.1243825

Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. / Sun, Yadong; Li, Lei; Macho, Alberto P.; Han, Zhifu; Hu, Zehan; Zipfel, Cyril; Zhou, Jian Min; Chai, Jijie.

In: Science, Vol. 342, No. 6158, 01.01.2013, p. 624-628.

Research output: Contribution to journalArticle

Sun, Y, Li, L, Macho, AP, Han, Z, Hu, Z, Zipfel, C, Zhou, JM & Chai, J 2013, 'Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex', Science, vol. 342, no. 6158, pp. 624-628. https://doi.org/10.1126/science.1243825
Sun, Yadong ; Li, Lei ; Macho, Alberto P. ; Han, Zhifu ; Hu, Zehan ; Zipfel, Cyril ; Zhou, Jian Min ; Chai, Jijie. / Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. In: Science. 2013 ; Vol. 342, No. 6158. pp. 624-628.
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