Structural Basis for Inactivation of the Human Pyruvate Dehydrogenase Complex by Phosphorylation: Role of Disordered Phosphorylation Loops

Masato Kato, R. Max Wynn, Jacinta L. Chuang, Shih Chia Tso, Mischa Machius, Jun Li, David T. Chuang

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

We report the crystal structures of the phosporylated pyruvate dehydrogenase (E1p) component of the human pyruvate dehydrogenase complex (PDC). The complete phosphorylation at Ser264-α (site 1) of a variant E1p protein was achieved using robust pyruvate dehydrogenase kinase 4 free of the PDC core. We show that unlike its unmodified counterpart, the presence of a phosphoryl group at Ser264-α prevents the cofactor thiamine diphosphate-induced ordering of the two loops carrying the three phosphorylation sites. The disordering of these phosphorylation loops is caused by a previously unrecognized steric clash between the phosphoryl group at site 1 and a nearby Ser266-α, which nullifies a hydrogen-bonding network essential for maintaining the loop conformations. The disordered phosphorylation loops impede the binding of lipoyl domains of the PDC core to E1p, negating the reductive acetylation step. This results in the disruption of the substrate channeling in the PDC, leading to the inactivation of this catalytic machine.

Original languageEnglish (US)
Pages (from-to)1849-1859
Number of pages11
JournalStructure
Volume16
Issue number12
DOIs
StatePublished - Dec 12 2008

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Pyruvate Dehydrogenase Complex
Phosphorylation
Thiamine Pyrophosphate
Hydrogen Bonding
Acetylation
Pyruvic Acid
Oxidoreductases
Proteins

Keywords

  • PROTEINS

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

Cite this

Structural Basis for Inactivation of the Human Pyruvate Dehydrogenase Complex by Phosphorylation : Role of Disordered Phosphorylation Loops. / Kato, Masato; Wynn, R. Max; Chuang, Jacinta L.; Tso, Shih Chia; Machius, Mischa; Li, Jun; Chuang, David T.

In: Structure, Vol. 16, No. 12, 12.12.2008, p. 1849-1859.

Research output: Contribution to journalArticle

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