Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex

Susanne Stefer, Simon Reitz, Fei Wang, Klemens Wild, Yin Yuin Pang, Daniel Schwarz, Jörg Bomke, Christopher Hein, Frank Löhr, Frank Bernhard, Vladimir Denic, Volker Dötsch, Irmgard Sinning

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Tail-anchored (TA) proteins are involved in cellular processes including trafficking, degradation, and apoptosis. They contain a C-terminal membrane anchor and are posttranslationally delivered to the endoplasmic reticulum (ER) membrane by the Get3 adenosine triphosphatase interacting with the hetero-oligomeric Get1/2 receptor. We have determined crystal structures of Get3 in complex with the cytosolic domains of Get1 and Get2 in different functional states at 3.0, 3.2, and 4.6 angstrom resolution. The structural data, together with biochemical experiments, show that Get1 and Get2 use adjacent, partially overlapping binding sites and that both can bind simultaneously to Get3. Docking to the Get1/2 complex allows for conformational changes in Get3 that are required for TA protein insertion. These data suggest a molecular mechanism for nucleotide-regulated delivery of TA proteins.

Original languageEnglish (US)
Pages (from-to)758-762
Number of pages5
JournalScience
Volume333
Issue number6043
DOIs
StatePublished - Aug 5 2011

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Membrane Proteins
Proteins
Membranes
Endoplasmic Reticulum
Adenosine Triphosphatases
Nucleotides
Binding Sites
Apoptosis

ASJC Scopus subject areas

  • General

Cite this

Stefer, S., Reitz, S., Wang, F., Wild, K., Pang, Y. Y., Schwarz, D., ... Sinning, I. (2011). Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex. Science, 333(6043), 758-762. https://doi.org/10.1126/science.1207125

Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex. / Stefer, Susanne; Reitz, Simon; Wang, Fei; Wild, Klemens; Pang, Yin Yuin; Schwarz, Daniel; Bomke, Jörg; Hein, Christopher; Löhr, Frank; Bernhard, Frank; Denic, Vladimir; Dötsch, Volker; Sinning, Irmgard.

In: Science, Vol. 333, No. 6043, 05.08.2011, p. 758-762.

Research output: Contribution to journalArticle

Stefer, S, Reitz, S, Wang, F, Wild, K, Pang, YY, Schwarz, D, Bomke, J, Hein, C, Löhr, F, Bernhard, F, Denic, V, Dötsch, V & Sinning, I 2011, 'Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex', Science, vol. 333, no. 6043, pp. 758-762. https://doi.org/10.1126/science.1207125
Stefer, Susanne ; Reitz, Simon ; Wang, Fei ; Wild, Klemens ; Pang, Yin Yuin ; Schwarz, Daniel ; Bomke, Jörg ; Hein, Christopher ; Löhr, Frank ; Bernhard, Frank ; Denic, Vladimir ; Dötsch, Volker ; Sinning, Irmgard. / Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex. In: Science. 2011 ; Vol. 333, No. 6043. pp. 758-762.
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AU - Schwarz, Daniel

AU - Bomke, Jörg

AU - Hein, Christopher

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