Structural basis for the activation of glycogen phosphorylase b by adenosine monophosphate

Stephen R. Sprang, Stephen G. Withers, Elizabeth J. Goldsmith, Robert J. Fletterick, Neil B. Madsen

Research output: Contribution to journalArticle

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Abstract

The three-dimensional structure of the activated state of glycogen phosphorylase (GP) as induced by adenosine monophosphate (AMP) has been determined from crystals of pyridoxalpyrophosphoryl-GP. The same quaternary changes relative to the inactive conformation as those induced by phosphorylation are induced by AMP, although the two regulatory signals function through different local structural mechanisms. Moreover, previous descriptions of the phosphorylase active state have been extended by demonstrating that, on activation, the amino- and carboxyl-terminal domains of GP rotate apart by 5°, thereby increasing access of substrates to the catalytic site. The structure also reveals previously unobserved interactions with the nucleotide that accounts for the specificity of the nucleotide binding site for AMP in preference to inosine monophosphate.

Original languageEnglish (US)
Pages (from-to)1367-1371
Number of pages5
JournalScience
Volume254
Issue number5036
StatePublished - Nov 29 1991

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Phosphorylase b
Glycogen Phosphorylase
Adenosine Monophosphate
Nucleotides
Inosine Monophosphate
Phosphorylases
Catalytic Domain
Binding Sites
Phosphorylation

ASJC Scopus subject areas

  • General

Cite this

Sprang, S. R., Withers, S. G., Goldsmith, E. J., Fletterick, R. J., & Madsen, N. B. (1991). Structural basis for the activation of glycogen phosphorylase b by adenosine monophosphate. Science, 254(5036), 1367-1371.

Structural basis for the activation of glycogen phosphorylase b by adenosine monophosphate. / Sprang, Stephen R.; Withers, Stephen G.; Goldsmith, Elizabeth J.; Fletterick, Robert J.; Madsen, Neil B.

In: Science, Vol. 254, No. 5036, 29.11.1991, p. 1367-1371.

Research output: Contribution to journalArticle

Sprang, SR, Withers, SG, Goldsmith, EJ, Fletterick, RJ & Madsen, NB 1991, 'Structural basis for the activation of glycogen phosphorylase b by adenosine monophosphate', Science, vol. 254, no. 5036, pp. 1367-1371.
Sprang SR, Withers SG, Goldsmith EJ, Fletterick RJ, Madsen NB. Structural basis for the activation of glycogen phosphorylase b by adenosine monophosphate. Science. 1991 Nov 29;254(5036):1367-1371.
Sprang, Stephen R. ; Withers, Stephen G. ; Goldsmith, Elizabeth J. ; Fletterick, Robert J. ; Madsen, Neil B. / Structural basis for the activation of glycogen phosphorylase b by adenosine monophosphate. In: Science. 1991 ; Vol. 254, No. 5036. pp. 1367-1371.
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