Structural basis of a phototropin light switch

Shannon M. Harper, Lori C. Neil, Kevin H. Gardner

Research output: Contribution to journalArticle

551 Scopus citations

Abstract

Phototropins are light-activated kinases important for plant responses to blue light. Light initiates signaling in these proteins by generating a covalent protein-flavin mononucleotide (FMN) adduct within sensory Per-ARNT-Sim (PAS) domains. We characterized the light-dependent changes of a phototropin PAS domain by solution nuclear magnetic resonance spectroscopy and found that an α helix located outside the canonical domain plays a key role in this activation process. Although this helix associates with the PAS core in the dark, photoinduced changes in the domain structure disrupt this interaction. We propose that this mechanism couples light-dependent bond formation to kinase activation and identifies a signaling pathway conserved among PAS domains.

Original languageEnglish (US)
Pages (from-to)1541-1544
Number of pages4
JournalScience
Volume301
Issue number5639
DOIs
StatePublished - Sep 12 2003

ASJC Scopus subject areas

  • General

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