TY - JOUR
T1 - Structural basis of eukaryotic gene transcription
AU - Boeger, Hinrich
AU - Bushnell, David A.
AU - Davis, Ralph
AU - Griesenbeck, Joachim
AU - Lorch, Yahli
AU - Strattan, J. Seth
AU - Westover, Kenneth D.
AU - Kornberg, Roger D.
PY - 2005/2/7
Y1 - 2005/2/7
N2 - An RNA polymerase II promoter has been isolated in transcriptionally activated and repressed states. Topological and nuclease digestion analyses have revealed a dynamic equilibrium between nucleosome removal and reassembly upon transcriptional activation, and have further shown that nucleosomes are removed by eviction of histone octamers rather than by sliding. The promoter, once exposed, assembles with RNA polymerase II, general transcription factors, and Mediator in a ∼3 MDa transcription initiation complex. X-ray crystallography has revealed the structure of RNA polymerase II, in the act of transcription, at atomic resolution. Extension of this analysis has shown how nucleotides undergo selection, polymerization, and eventual release from the transcribing complex. X-ray and electron crystallography have led to a picture of the entire transcription initiation complex, elucidating the mechanisms of promoter recognition, DNA unwinding, abortive initiation, and promoter escape.
AB - An RNA polymerase II promoter has been isolated in transcriptionally activated and repressed states. Topological and nuclease digestion analyses have revealed a dynamic equilibrium between nucleosome removal and reassembly upon transcriptional activation, and have further shown that nucleosomes are removed by eviction of histone octamers rather than by sliding. The promoter, once exposed, assembles with RNA polymerase II, general transcription factors, and Mediator in a ∼3 MDa transcription initiation complex. X-ray crystallography has revealed the structure of RNA polymerase II, in the act of transcription, at atomic resolution. Extension of this analysis has shown how nucleotides undergo selection, polymerization, and eventual release from the transcribing complex. X-ray and electron crystallography have led to a picture of the entire transcription initiation complex, elucidating the mechanisms of promoter recognition, DNA unwinding, abortive initiation, and promoter escape.
KW - General transcription factors
KW - Mediator
KW - Nucleosome
KW - RNA polymerase II
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U2 - 10.1016/j.febslet.2004.11.027
DO - 10.1016/j.febslet.2004.11.027
M3 - Article
C2 - 15680971
AN - SCOPUS:14544293651
SN - 0014-5793
VL - 579
SP - 899
EP - 903
JO - FEBS Letters
JF - FEBS Letters
IS - 4 SPEC. ISS.
ER -