Structural basis of gating by the outer membrane transporter FecA

Andrew D. Ferguson, Ranjan Chakraborty, Barbara S. Smith, Lothar Esser, Dick Van Der Helm, Johann Deisenhofer

Research output: Contribution to journalArticle

282 Citations (Scopus)

Abstract

Siderophore-mediated acquisition systems facilitate iron uptake. We present the crystallographic structure of the integral outer membrane receptor FecA from Escherichia coli with and without ferric citrate at 2.5 and 2.0 angstrom resolution. FecA is composed of three distinct domains: the barrel, plug, and NH2- terminal extension. Binding of ferric citrate triggers a conformational change of the extracellular loops that close the external pocket of FecA. Ligand-induced allosteric transitions are propagated through the outer membrane by the plug domain, signaling the occupancy of the receptor in the periplasm. These data establish the structural basis of gating for receptors dependent on the cytoplasmic membrane protein TonB. By compiling available data for this family of receptors, we propose a mechanism for the energy-dependent transport of siderophores.

Original languageEnglish (US)
Pages (from-to)1715-1719
Number of pages5
JournalScience
Volume295
Issue number5560
DOIs
StatePublished - Mar 1 2002

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Siderophores
Membrane Transport Proteins
Periplasm
Membranes
Membrane Proteins
Iron
Cell Membrane
Escherichia coli
Ligands
ferric citrate

ASJC Scopus subject areas

  • General

Cite this

Ferguson, A. D., Chakraborty, R., Smith, B. S., Esser, L., Van Der Helm, D., & Deisenhofer, J. (2002). Structural basis of gating by the outer membrane transporter FecA. Science, 295(5560), 1715-1719. https://doi.org/10.1126/science.1067313

Structural basis of gating by the outer membrane transporter FecA. / Ferguson, Andrew D.; Chakraborty, Ranjan; Smith, Barbara S.; Esser, Lothar; Van Der Helm, Dick; Deisenhofer, Johann.

In: Science, Vol. 295, No. 5560, 01.03.2002, p. 1715-1719.

Research output: Contribution to journalArticle

Ferguson, AD, Chakraborty, R, Smith, BS, Esser, L, Van Der Helm, D & Deisenhofer, J 2002, 'Structural basis of gating by the outer membrane transporter FecA', Science, vol. 295, no. 5560, pp. 1715-1719. https://doi.org/10.1126/science.1067313
Ferguson AD, Chakraborty R, Smith BS, Esser L, Van Der Helm D, Deisenhofer J. Structural basis of gating by the outer membrane transporter FecA. Science. 2002 Mar 1;295(5560):1715-1719. https://doi.org/10.1126/science.1067313
Ferguson, Andrew D. ; Chakraborty, Ranjan ; Smith, Barbara S. ; Esser, Lothar ; Van Der Helm, Dick ; Deisenhofer, Johann. / Structural basis of gating by the outer membrane transporter FecA. In: Science. 2002 ; Vol. 295, No. 5560. pp. 1715-1719.
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