Structural basis of photosensitivity in a bacterial light-oxygen-voltage/ helix-turn-helix (LOV-HTH) DNA-binding protein

Abigail I. Nash, Reginald McNulty, Mary Elizabeth Shillito, Trevor E. Swartz, Roberto A. Bogomolni, Hartmut Luecke, Kevin H. Gardner

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Light-oxygen-voltage (LOV) domains are blue light-activated signaling modules integral to a wide range of photosensory proteins. Upon illumination, LOV domains form internal protein-flavin adducts that generate conformational changes which control effector function. Here we advance our understanding of LOV regulation with structural, biophysical, and biochemical studies of EL222, a light-regulated DNA-binding protein. The dark-state crystal structure reveals interactions between the EL222 LOV and helix-turn-helix domains that we show inhibit DNA binding. Solution biophysical data indicate that illumination breaks these interactions, freeing the LOV and helix-turn-helix domains of each other. This conformational change has a key functional effect, allowing EL222 to bind DNA in a light-dependent manner. Our data reveal a conserved signaling mechanism among diverse LOV-containing proteins, where light-induced conformational changes trigger activation via a conserved interaction surface.

Original languageEnglish (US)
Pages (from-to)9449-9454
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number23
DOIs
StatePublished - Jun 7 2011

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DNA-Binding Proteins
Oxygen
Light
Lighting
Proteins
DNA

Keywords

  • Allosteric regulation
  • PER-ARNT-SIM domain
  • Photosensing

ASJC Scopus subject areas

  • General

Cite this

Structural basis of photosensitivity in a bacterial light-oxygen-voltage/ helix-turn-helix (LOV-HTH) DNA-binding protein. / Nash, Abigail I.; McNulty, Reginald; Shillito, Mary Elizabeth; Swartz, Trevor E.; Bogomolni, Roberto A.; Luecke, Hartmut; Gardner, Kevin H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, No. 23, 07.06.2011, p. 9449-9454.

Research output: Contribution to journalArticle

Nash, Abigail I. ; McNulty, Reginald ; Shillito, Mary Elizabeth ; Swartz, Trevor E. ; Bogomolni, Roberto A. ; Luecke, Hartmut ; Gardner, Kevin H. / Structural basis of photosensitivity in a bacterial light-oxygen-voltage/ helix-turn-helix (LOV-HTH) DNA-binding protein. In: Proceedings of the National Academy of Sciences of the United States of America. 2011 ; Vol. 108, No. 23. pp. 9449-9454.
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