Structural Basis of Transcription

Role of the Trigger Loop in Substrate Specificity and Catalysis

Dong Wang, David A. Bushnell, Kenneth D. Westover, Craig D. Kaplan, Roger D. Kornberg

Research output: Contribution to journalArticle

305 Citations (Scopus)

Abstract

New structures of RNA polymerase II (pol II) transcribing complexes reveal a likely key to transcription. The trigger loop swings beneath a correct nucleoside triphosphate (NTP) in the nucleotide addition site, closing off the active center and forming an extensive network of interactions with the NTP base, sugar, phosphates, and additional pol II residues. A histidine side chain in the trigger loop, precisely positioned by these interactions, may literally "trigger" phosphodiester bond formation. Recognition and catalysis are thus coupled, ensuring the fidelity of transcription.

Original languageEnglish (US)
Pages (from-to)941-954
Number of pages14
JournalCell
Volume127
Issue number5
DOIs
StatePublished - Dec 1 2006

Fingerprint

Transcription
Substrate Specificity
Catalysis
Nucleosides
Sugar Phosphates
RNA Polymerase II
Substrates
Histidine
Catalytic Domain
Nucleotides
triphosphoric acid

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Structural Basis of Transcription : Role of the Trigger Loop in Substrate Specificity and Catalysis. / Wang, Dong; Bushnell, David A.; Westover, Kenneth D.; Kaplan, Craig D.; Kornberg, Roger D.

In: Cell, Vol. 127, No. 5, 01.12.2006, p. 941-954.

Research output: Contribution to journalArticle

Wang, Dong ; Bushnell, David A. ; Westover, Kenneth D. ; Kaplan, Craig D. ; Kornberg, Roger D. / Structural Basis of Transcription : Role of the Trigger Loop in Substrate Specificity and Catalysis. In: Cell. 2006 ; Vol. 127, No. 5. pp. 941-954.
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