Structural characterization of full-length NSF and 20S particles

Lei Fu Chang, Song Chen, Cui Cui Liu, Xijiang Pan, Jiansen Jiang, Xiao Chen Bai, Xin Xie, Hong Wei Wang, Sen Fang Sui

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The 20S particle, which is composed of the N-ethylmaleimide-sensitive factor (NSF), soluble NSF attachment proteins (SNAPs) and the SNAP receptor (SNARE) complex, has an essential role in intracellular vesicle fusion events. Using single-particle cryo-EM and negative stain EM, we reconstructed four related three-dimensional structures: Chinese hamster NSF hexamer in the ATPγS, ADP-AlFx and ADP states, and the 20S particle. These structures reveal a parallel arrangement between the D1 and D2 domains of the hexameric NSF and characterize the nucleotide-dependent conformational changes in NSF. The structure of the 20S particle shows that it holds the SNARE complex at two interaction interfaces around the C terminus and N-terminal half of the SNARE complex, respectively. These findings provide insight into the molecular mechanism underlying disassembly of the SNARE complex by NSF.

Original languageEnglish (US)
Pages (from-to)268-275
Number of pages8
JournalNature Structural and Molecular Biology
Volume19
Issue number3
DOIs
StatePublished - Mar 1 2012

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N-Ethylmaleimide-Sensitive Proteins
SNARE Proteins
Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
Adenosine Diphosphate
Cricetulus
Coloring Agents
Nucleotides

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Chang, L. F., Chen, S., Liu, C. C., Pan, X., Jiang, J., Bai, X. C., ... Sui, S. F. (2012). Structural characterization of full-length NSF and 20S particles. Nature Structural and Molecular Biology, 19(3), 268-275. https://doi.org/10.1038/nsmb.2237

Structural characterization of full-length NSF and 20S particles. / Chang, Lei Fu; Chen, Song; Liu, Cui Cui; Pan, Xijiang; Jiang, Jiansen; Bai, Xiao Chen; Xie, Xin; Wang, Hong Wei; Sui, Sen Fang.

In: Nature Structural and Molecular Biology, Vol. 19, No. 3, 01.03.2012, p. 268-275.

Research output: Contribution to journalArticle

Chang, LF, Chen, S, Liu, CC, Pan, X, Jiang, J, Bai, XC, Xie, X, Wang, HW & Sui, SF 2012, 'Structural characterization of full-length NSF and 20S particles', Nature Structural and Molecular Biology, vol. 19, no. 3, pp. 268-275. https://doi.org/10.1038/nsmb.2237
Chang, Lei Fu ; Chen, Song ; Liu, Cui Cui ; Pan, Xijiang ; Jiang, Jiansen ; Bai, Xiao Chen ; Xie, Xin ; Wang, Hong Wei ; Sui, Sen Fang. / Structural characterization of full-length NSF and 20S particles. In: Nature Structural and Molecular Biology. 2012 ; Vol. 19, No. 3. pp. 268-275.
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