Structural characterization of heme sites in spinach cytochrome b6f complexes: a resonance Raman study

J. David Hobbs; Max Wynn; David J. Nunez; Richard Malkin; David B. Knaff; Mark R. Ondrias

doi:10.1016/S0005-2728(05)80185-9

Structural characterization of heme sites in spinach cytochrome b₆f complexes: a resonance Raman study

J. David Hobbs, Max Wynn, David J. Nunez, Richard Malkin, David B. Knaff, Mark R. Ondrias

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Resonance Raman spectra of cytochrome b₆f complexes isolated from spinach chloroplasts have been obtained. Selective resonance enhancements and partial reductions of the complex by redox mediators were used to isolate and identify the contributions of heme b₆ and heme f sites to the observed spectra. Corresponding spectra for turnip cytochrome f have also been obtained. Power-dependent photoreduction was observed in cytochrome f of the complex as well as in the isolated cytochrome f during the course of the Raman experiments.

Original language	English (US)
Pages (from-to)	37-44
Number of pages	8
Journal	BBA - Bioenergetics
Volume	1059
Issue number	1
DOIs	https://doi.org/10.1016/S0005-2728(05)80185-9
State	Published - Aug 2 1991

Keywords

Cytochrome bf
Cytochrome f
Heme
Photoreduction
Resonance Raman

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

Access to Document

10.1016/S0005-2728(05)80185-9

Cite this

@article{7ef92d8d26a0482ab15ef744d1de6bee,

title = "Structural characterization of heme sites in spinach cytochrome b6f complexes: a resonance Raman study",

abstract = "Resonance Raman spectra of cytochrome b6f complexes isolated from spinach chloroplasts have been obtained. Selective resonance enhancements and partial reductions of the complex by redox mediators were used to isolate and identify the contributions of heme b6 and heme f sites to the observed spectra. Corresponding spectra for turnip cytochrome f have also been obtained. Power-dependent photoreduction was observed in cytochrome f of the complex as well as in the isolated cytochrome f during the course of the Raman experiments.",

keywords = "Cytochrome bf, Cytochrome f, Heme, Photoreduction, Resonance Raman",

author = "Hobbs, {J. David} and Max Wynn and Nunez, {David J.} and Richard Malkin and Knaff, {David B.} and Ondrias, {Mark R.}",

note = "Funding Information: The authors thank Dr. Kevin A. Gray for preparation of the cytochrome b6f complexes utilized in the early portions of this work. The financial support of the NSF (DMB-8716033 to D.B.K.), NIH (GM-20571 to R.M.), and the NIH (GM-33330 to M.R.O.) is gratefully acknowledged.",

year = "1991",

month = aug,

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doi = "10.1016/S0005-2728(05)80185-9",

language = "English (US)",

volume = "1059",

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TY - JOUR

T1 - Structural characterization of heme sites in spinach cytochrome b6f complexes

T2 - a resonance Raman study

AU - Hobbs, J. David

AU - Wynn, Max

AU - Nunez, David J.

AU - Malkin, Richard

AU - Knaff, David B.

AU - Ondrias, Mark R.

N1 - Funding Information: The authors thank Dr. Kevin A. Gray for preparation of the cytochrome b6f complexes utilized in the early portions of this work. The financial support of the NSF (DMB-8716033 to D.B.K.), NIH (GM-20571 to R.M.), and the NIH (GM-33330 to M.R.O.) is gratefully acknowledged.

PY - 1991/8/2

Y1 - 1991/8/2

N2 - Resonance Raman spectra of cytochrome b6f complexes isolated from spinach chloroplasts have been obtained. Selective resonance enhancements and partial reductions of the complex by redox mediators were used to isolate and identify the contributions of heme b6 and heme f sites to the observed spectra. Corresponding spectra for turnip cytochrome f have also been obtained. Power-dependent photoreduction was observed in cytochrome f of the complex as well as in the isolated cytochrome f during the course of the Raman experiments.

AB - Resonance Raman spectra of cytochrome b6f complexes isolated from spinach chloroplasts have been obtained. Selective resonance enhancements and partial reductions of the complex by redox mediators were used to isolate and identify the contributions of heme b6 and heme f sites to the observed spectra. Corresponding spectra for turnip cytochrome f have also been obtained. Power-dependent photoreduction was observed in cytochrome f of the complex as well as in the isolated cytochrome f during the course of the Raman experiments.

KW - Cytochrome bf

KW - Cytochrome f

KW - Heme

KW - Photoreduction

KW - Resonance Raman

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