Structural characterization of heme sites in spinach cytochrome b6f complexes: a resonance Raman study

J. David Hobbs, Max Wynn, David J. Nunez, Richard Malkin, David B. Knaff, Mark R. Ondrias

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Resonance Raman spectra of cytochrome b6f complexes isolated from spinach chloroplasts have been obtained. Selective resonance enhancements and partial reductions of the complex by redox mediators were used to isolate and identify the contributions of heme b6 and heme f sites to the observed spectra. Corresponding spectra for turnip cytochrome f have also been obtained. Power-dependent photoreduction was observed in cytochrome f of the complex as well as in the isolated cytochrome f during the course of the Raman experiments.

Original languageEnglish (US)
Pages (from-to)37-44
Number of pages8
JournalBBA - Bioenergetics
Volume1059
Issue number1
DOIs
StatePublished - Aug 2 1991

Fingerprint

Cytochrome b6f Complex
Cytochromes f
Spinacia oleracea
Heme
Mediator Complex
Brassica napus
Chloroplasts
Oxidation-Reduction
Raman scattering
Experiments

Keywords

  • Cytochrome bf
  • Cytochrome f
  • Heme
  • Photoreduction
  • Resonance Raman

ASJC Scopus subject areas

  • Biophysics

Cite this

Structural characterization of heme sites in spinach cytochrome b6f complexes : a resonance Raman study. / Hobbs, J. David; Wynn, Max; Nunez, David J.; Malkin, Richard; Knaff, David B.; Ondrias, Mark R.

In: BBA - Bioenergetics, Vol. 1059, No. 1, 02.08.1991, p. 37-44.

Research output: Contribution to journalArticle

Hobbs, J. David ; Wynn, Max ; Nunez, David J. ; Malkin, Richard ; Knaff, David B. ; Ondrias, Mark R. / Structural characterization of heme sites in spinach cytochrome b6f complexes : a resonance Raman study. In: BBA - Bioenergetics. 1991 ; Vol. 1059, No. 1. pp. 37-44.
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AU - Knaff, David B.

AU - Ondrias, Mark R.

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