Structural Characterization of Monohydroxyeicosatetraenoic Acids and Dihydroxy- and Trihydroxyeicosatrienoic Acids by ESI-FTICR

Lijie Cui, Marilyn A. Isbell, Yuttana Chawengsub, J R Falck, William B. Campbell, Kasem Nithipatikom

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The fragmentation characteristics of monohydroxyeicosatetraenoic acids and dihydroxy- and trihydroxyeicosatrienoic acids were investigated by electrospray ionization Fourier transform ion cyclotron resonance (FTICR) mass spectrometry using sustained off-resonance irradiation collision-induced dissociation (SORI-CID) and infrared multiphoton dissociation (IRMPD). The fragmentation patterns of these compounds were associated with the number and positions of the hydroxyl substituents. The fragmentation is more complicated with increasing number of the hydroxyl groups of the compounds. In general, the major carbon-carbon cleavage of [M - H]- ions occurred at the α-position to the hydroxyl group, and the carbon-carbon cleavage occurred when there was a double-bond at the β-position to the hydroxyl group. SORI-CID and IRMPD produced some common fragmentation patterns; however, each technique provided some unique patterns that are useful for structural identification of these compounds. This study demonstrated the application of FTICR via the identification of regioisomers of trihydroxyeicosatrienoic acids in rabbit aorta samples.

Original languageEnglish (US)
Pages (from-to)569-585
Number of pages17
JournalJournal of the American Society for Mass Spectrometry
Volume19
Issue number4
DOIs
StatePublished - Apr 2008

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Cyclotrons
Cyclotron resonance
Fourier Analysis
Hydroxyl Radical
Fourier transforms
Carbon
Ions
Acids
Irradiation
Infrared radiation
Electrospray ionization
Mass spectrometry
Aorta
Mass Spectrometry
Rabbits

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

Cite this

Structural Characterization of Monohydroxyeicosatetraenoic Acids and Dihydroxy- and Trihydroxyeicosatrienoic Acids by ESI-FTICR. / Cui, Lijie; Isbell, Marilyn A.; Chawengsub, Yuttana; Falck, J R; Campbell, William B.; Nithipatikom, Kasem.

In: Journal of the American Society for Mass Spectrometry, Vol. 19, No. 4, 04.2008, p. 569-585.

Research output: Contribution to journalArticle

Cui, Lijie ; Isbell, Marilyn A. ; Chawengsub, Yuttana ; Falck, J R ; Campbell, William B. ; Nithipatikom, Kasem. / Structural Characterization of Monohydroxyeicosatetraenoic Acids and Dihydroxy- and Trihydroxyeicosatrienoic Acids by ESI-FTICR. In: Journal of the American Society for Mass Spectrometry. 2008 ; Vol. 19, No. 4. pp. 569-585.
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