Structural determinants of the renal vascular response to 20-hydroxyeicosatetraenoic acid (20-HETE)

M. Alonso-Galicia, J R Falck, D. R. Harder, R. J. Roman

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

20-HETE is an endogenously-formed vasoconstrictor that plays a role in tubuloglomerular feedback and autoregulation of renal blood flow. However, little is known about the mechanism of action of this compound or whether a receptor is involved. The present study compared the effects of 20-HETE and a series of synthetic analogues on vascular diameter of isolated perfused renal arterioles of the rat to determine the structural requirements to elevate vascular tone. Cumulative dose-response relationships to 5-, 12-. 15-, 19-, 20-, 21-HETEs, and a dimethyl derivative of 20-HETE were determined in renal interlobular arteries (100-150 μm) treated with indomethacin (5 μM), baicalein (0.5 μM), and 17-ODYA (20 μM) to block the endogenous production of arachidonic acid metabolites. 20-HETE (0.01 to 1 μM) reduced the diameter of these vessels in a concentration dependent manner by a maximum of 18±3% (n=6 vessels). 21-HETE and dimethyl 20-HETE had a similar effect and reduced vascular diameter by a maximum of 16±3% (n=5) and 15±2% (n=6). respectively. In contrast, 5-, 12-, 15-, or 19-HETE had no effect on vascular diameter. Moreover, pretreatment of the vessels with 5-, 15-or 19-HETE(1 μM) blocked the vasoconstrictor response to 20-HETE. These results suggest that the vasoconstrictor response to HETEs is dependent on the presence of a hydroxyl group on the 20 or 21 carbon while 5-, 15- and 19-HETE act as antagonists to the vasoconstrictor response to 20-HETE.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997

Fingerprint

blood vessels
Blood Vessels
vasoconstrictor agents
kidneys
Kidney
Vasoconstrictor Agents
acids
Hydroxyeicosatetraenoic Acids
autoregulation
indomethacin
Metabolites
20-hydroxy-5,8,11,14-eicosatetraenoic acid
Renal Circulation
arachidonic acid
Arachidonic Acid
Indomethacin
Hydroxyl Radical
Arterioles
Renal Artery
arteries

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Structural determinants of the renal vascular response to 20-hydroxyeicosatetraenoic acid (20-HETE). / Alonso-Galicia, M.; Falck, J R; Harder, D. R.; Roman, R. J.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

@article{b95bfd8aa07c4ebab44d384906a1272f,
title = "Structural determinants of the renal vascular response to 20-hydroxyeicosatetraenoic acid (20-HETE)",
abstract = "20-HETE is an endogenously-formed vasoconstrictor that plays a role in tubuloglomerular feedback and autoregulation of renal blood flow. However, little is known about the mechanism of action of this compound or whether a receptor is involved. The present study compared the effects of 20-HETE and a series of synthetic analogues on vascular diameter of isolated perfused renal arterioles of the rat to determine the structural requirements to elevate vascular tone. Cumulative dose-response relationships to 5-, 12-. 15-, 19-, 20-, 21-HETEs, and a dimethyl derivative of 20-HETE were determined in renal interlobular arteries (100-150 μm) treated with indomethacin (5 μM), baicalein (0.5 μM), and 17-ODYA (20 μM) to block the endogenous production of arachidonic acid metabolites. 20-HETE (0.01 to 1 μM) reduced the diameter of these vessels in a concentration dependent manner by a maximum of 18±3{\%} (n=6 vessels). 21-HETE and dimethyl 20-HETE had a similar effect and reduced vascular diameter by a maximum of 16±3{\%} (n=5) and 15±2{\%} (n=6). respectively. In contrast, 5-, 12-, 15-, or 19-HETE had no effect on vascular diameter. Moreover, pretreatment of the vessels with 5-, 15-or 19-HETE(1 μM) blocked the vasoconstrictor response to 20-HETE. These results suggest that the vasoconstrictor response to HETEs is dependent on the presence of a hydroxyl group on the 20 or 21 carbon while 5-, 15- and 19-HETE act as antagonists to the vasoconstrictor response to 20-HETE.",
author = "M. Alonso-Galicia and Falck, {J R} and Harder, {D. R.} and Roman, {R. J.}",
year = "1997",
language = "English (US)",
volume = "11",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "3",

}

TY - JOUR

T1 - Structural determinants of the renal vascular response to 20-hydroxyeicosatetraenoic acid (20-HETE)

AU - Alonso-Galicia, M.

AU - Falck, J R

AU - Harder, D. R.

AU - Roman, R. J.

PY - 1997

Y1 - 1997

N2 - 20-HETE is an endogenously-formed vasoconstrictor that plays a role in tubuloglomerular feedback and autoregulation of renal blood flow. However, little is known about the mechanism of action of this compound or whether a receptor is involved. The present study compared the effects of 20-HETE and a series of synthetic analogues on vascular diameter of isolated perfused renal arterioles of the rat to determine the structural requirements to elevate vascular tone. Cumulative dose-response relationships to 5-, 12-. 15-, 19-, 20-, 21-HETEs, and a dimethyl derivative of 20-HETE were determined in renal interlobular arteries (100-150 μm) treated with indomethacin (5 μM), baicalein (0.5 μM), and 17-ODYA (20 μM) to block the endogenous production of arachidonic acid metabolites. 20-HETE (0.01 to 1 μM) reduced the diameter of these vessels in a concentration dependent manner by a maximum of 18±3% (n=6 vessels). 21-HETE and dimethyl 20-HETE had a similar effect and reduced vascular diameter by a maximum of 16±3% (n=5) and 15±2% (n=6). respectively. In contrast, 5-, 12-, 15-, or 19-HETE had no effect on vascular diameter. Moreover, pretreatment of the vessels with 5-, 15-or 19-HETE(1 μM) blocked the vasoconstrictor response to 20-HETE. These results suggest that the vasoconstrictor response to HETEs is dependent on the presence of a hydroxyl group on the 20 or 21 carbon while 5-, 15- and 19-HETE act as antagonists to the vasoconstrictor response to 20-HETE.

AB - 20-HETE is an endogenously-formed vasoconstrictor that plays a role in tubuloglomerular feedback and autoregulation of renal blood flow. However, little is known about the mechanism of action of this compound or whether a receptor is involved. The present study compared the effects of 20-HETE and a series of synthetic analogues on vascular diameter of isolated perfused renal arterioles of the rat to determine the structural requirements to elevate vascular tone. Cumulative dose-response relationships to 5-, 12-. 15-, 19-, 20-, 21-HETEs, and a dimethyl derivative of 20-HETE were determined in renal interlobular arteries (100-150 μm) treated with indomethacin (5 μM), baicalein (0.5 μM), and 17-ODYA (20 μM) to block the endogenous production of arachidonic acid metabolites. 20-HETE (0.01 to 1 μM) reduced the diameter of these vessels in a concentration dependent manner by a maximum of 18±3% (n=6 vessels). 21-HETE and dimethyl 20-HETE had a similar effect and reduced vascular diameter by a maximum of 16±3% (n=5) and 15±2% (n=6). respectively. In contrast, 5-, 12-, 15-, or 19-HETE had no effect on vascular diameter. Moreover, pretreatment of the vessels with 5-, 15-or 19-HETE(1 μM) blocked the vasoconstrictor response to 20-HETE. These results suggest that the vasoconstrictor response to HETEs is dependent on the presence of a hydroxyl group on the 20 or 21 carbon while 5-, 15- and 19-HETE act as antagonists to the vasoconstrictor response to 20-HETE.

UR - http://www.scopus.com/inward/record.url?scp=17544363192&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=17544363192&partnerID=8YFLogxK

M3 - Article

VL - 11

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 3

ER -