Abstract
Molecules specified by the H 2 linked T/t locus in the mouse are expressed on H 2 negative cells such as early embryos, sperm, and teratoma cells. By means of enzymatic radioiodination of cells and immunoprecipitation of lysates with congenic antiserum, one of these molecules, known as F9, has been obtained from sperm and teratoma cells and compared to H 2 isolated from murine splenocytes. Our studies indicate that both H 2 and F9 have identical molecular weights and subunit structure, including the presence of a B2 microglobulin like subunit. These findings, taken together with previous studies of TL alloantigens, suggest that a number of gene products of the 9th linkage group show structural homology with each other and, in addition, with immunoglobulin. The genes in question may therefore have arisen from a primitive gene concerned with cellular recognition.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3215-3219 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 72 |
| Issue number | 8 |
| DOIs | |
| State | Published - 1975 |
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ASJC Scopus subject areas
- Genetics
- General
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Structural similarities between a product of the T/t locus isolated from sperm and teratoma cells, and H 2 antigens isolated from splenocytes. / Vitetta, E. S.; Artzt, K.; Bennett, D.; Boyse, E. A.; Jacob, F.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 72, No. 8, 1975, p. 3215-3219.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Structural similarities between a product of the T/t locus isolated from sperm and teratoma cells, and H 2 antigens isolated from splenocytes
AU - Vitetta, E. S.
AU - Artzt, K.
AU - Bennett, D.
AU - Boyse, E. A.
AU - Jacob, F.
PY - 1975
Y1 - 1975
N2 - Molecules specified by the H 2 linked T/t locus in the mouse are expressed on H 2 negative cells such as early embryos, sperm, and teratoma cells. By means of enzymatic radioiodination of cells and immunoprecipitation of lysates with congenic antiserum, one of these molecules, known as F9, has been obtained from sperm and teratoma cells and compared to H 2 isolated from murine splenocytes. Our studies indicate that both H 2 and F9 have identical molecular weights and subunit structure, including the presence of a B2 microglobulin like subunit. These findings, taken together with previous studies of TL alloantigens, suggest that a number of gene products of the 9th linkage group show structural homology with each other and, in addition, with immunoglobulin. The genes in question may therefore have arisen from a primitive gene concerned with cellular recognition.
AB - Molecules specified by the H 2 linked T/t locus in the mouse are expressed on H 2 negative cells such as early embryos, sperm, and teratoma cells. By means of enzymatic radioiodination of cells and immunoprecipitation of lysates with congenic antiserum, one of these molecules, known as F9, has been obtained from sperm and teratoma cells and compared to H 2 isolated from murine splenocytes. Our studies indicate that both H 2 and F9 have identical molecular weights and subunit structure, including the presence of a B2 microglobulin like subunit. These findings, taken together with previous studies of TL alloantigens, suggest that a number of gene products of the 9th linkage group show structural homology with each other and, in addition, with immunoglobulin. The genes in question may therefore have arisen from a primitive gene concerned with cellular recognition.
UR - http://www.scopus.com/inward/record.url?scp=0016707617&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0016707617&partnerID=8YFLogxK
U2 - 10.1073/pnas.72.8.3215
DO - 10.1073/pnas.72.8.3215
M3 - Article
C2 - 1059107
AN - SCOPUS:0016707617
VL - 72
SP - 3215
EP - 3219
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 8
ER -