Structural studies of cell surface and secreted IgG in LPS-stimulated murine B cells

Dorothy Yuan; Ellen S. Vitetta

doi:10.1016/0161-5890(83)90018-4

Structural studies of cell surface and secreted IgG in LPS-stimulated murine B cells

Dorothy Yuan, Ellen S. Vitetta

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

We have characterized the cell surface IgG of LPS-stimulated B lymphocytes by surface iodination and specific immunoprecipitation. IgG₃ was found to be the predominant IgG subclass expressed on the B cell surface after 5 days of stimulation with LPS. In contrast. IgG_2b, IgG₃, and IgG₁ subclasses are all secreted. Cell surface IgG exhibits detergent binding characteristics not shown by secreted IgG and the y chains of the three subclasses of surface IgG examined have the apparent mol. wt of 64 Kd. some 9.000 larger than the H chains of secreted IgG.

Original language	English (US)
Pages (from-to)	367-375
Number of pages	9
Journal	Molecular Immunology
Volume	20
Issue number	4
DOIs	https://doi.org/10.1016/0161-5890(83)90018-4
State	Published - Apr 1983

ASJC Scopus subject areas

Immunology
Molecular Biology

Access to Document

10.1016/0161-5890(83)90018-4

Cite this

@article{b23aa1bb1d974835863ee50516cdd924,

title = "Structural studies of cell surface and secreted IgG in LPS-stimulated murine B cells",

abstract = "We have characterized the cell surface IgG of LPS-stimulated B lymphocytes by surface iodination and specific immunoprecipitation. IgG3 was found to be the predominant IgG subclass expressed on the B cell surface after 5 days of stimulation with LPS. In contrast. IgG2b, IgG3, and IgG1 subclasses are all secreted. Cell surface IgG exhibits detergent binding characteristics not shown by secreted IgG and the y chains of the three subclasses of surface IgG examined have the apparent mol. wt of 64 Kd. some 9.000 larger than the H chains of secreted IgG.",

author = "Dorothy Yuan and Vitetta, {Ellen S.}",

year = "1983",

month = apr,

doi = "10.1016/0161-5890(83)90018-4",

language = "English (US)",

volume = "20",

pages = "367--375",

journal = "Molecular Immunology",

issn = "0161-5890",

publisher = "Elsevier Limited",

number = "4",

}

TY - JOUR

T1 - Structural studies of cell surface and secreted IgG in LPS-stimulated murine B cells

AU - Yuan, Dorothy

AU - Vitetta, Ellen S.

PY - 1983/4

Y1 - 1983/4

N2 - We have characterized the cell surface IgG of LPS-stimulated B lymphocytes by surface iodination and specific immunoprecipitation. IgG3 was found to be the predominant IgG subclass expressed on the B cell surface after 5 days of stimulation with LPS. In contrast. IgG2b, IgG3, and IgG1 subclasses are all secreted. Cell surface IgG exhibits detergent binding characteristics not shown by secreted IgG and the y chains of the three subclasses of surface IgG examined have the apparent mol. wt of 64 Kd. some 9.000 larger than the H chains of secreted IgG.

AB - We have characterized the cell surface IgG of LPS-stimulated B lymphocytes by surface iodination and specific immunoprecipitation. IgG3 was found to be the predominant IgG subclass expressed on the B cell surface after 5 days of stimulation with LPS. In contrast. IgG2b, IgG3, and IgG1 subclasses are all secreted. Cell surface IgG exhibits detergent binding characteristics not shown by secreted IgG and the y chains of the three subclasses of surface IgG examined have the apparent mol. wt of 64 Kd. some 9.000 larger than the H chains of secreted IgG.

UR - http://www.scopus.com/inward/record.url?scp=0020600259&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020600259&partnerID=8YFLogxK

U2 - 10.1016/0161-5890(83)90018-4

DO - 10.1016/0161-5890(83)90018-4

M3 - Article

C2 - 6191206

AN - SCOPUS:0020600259

SN - 0161-5890

VL - 20

SP - 367

EP - 375

JO - Molecular Immunology

JF - Molecular Immunology

IS - 4

ER -

Structural studies of cell surface and secreted IgG in LPS-stimulated murine B cells

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this