Structural studies of cell surface and secreted IgG in LPS-stimulated murine B cells

Dorothy Yuan, Ellen S. Vitetta

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24 Scopus citations

Abstract

We have characterized the cell surface IgG of LPS-stimulated B lymphocytes by surface iodination and specific immunoprecipitation. IgG3 was found to be the predominant IgG subclass expressed on the B cell surface after 5 days of stimulation with LPS. In contrast. IgG2b, IgG3, and IgG1 subclasses are all secreted. Cell surface IgG exhibits detergent binding characteristics not shown by secreted IgG and the y chains of the three subclasses of surface IgG examined have the apparent mol. wt of 64 Kd. some 9.000 larger than the H chains of secreted IgG.

Original languageEnglish (US)
Pages (from-to)367-375
Number of pages9
JournalMolecular Immunology
Volume20
Issue number4
DOIs
StatePublished - Apr 1983

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ASJC Scopus subject areas

  • Immunology
  • Molecular Biology

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