Structural studies of MAP Kinase cascade components.

Elizabeth J. Goldsmith, Xiaoshan Min, Haixia He, Tianjun Zhou

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

MAPK cascade components have been the subject of structural analysis, advancing our understanding of how these enzymes are activated and how they interact. A surprising finding has been that unique inactive conformers are adopted by many of these kinases. These inactive conformers are interesting and often require experimental phases to determine their crystal structures because molecular replacement techniques are not successful. Here, we describe the preparation of MAP2K MEK6 and MAP3K TAO2 substituted with selenomethionine (SeMet) for de novo phasing. TAO2 and SeMet TAO2 were expressed in insect cells.

Original languageEnglish (US)
Pages (from-to)223-237
Number of pages15
JournalMethods in molecular biology (Clifton, N.J.)
Volume661
StatePublished - 2010

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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