Structure and biosynthesis of the BT peptide antibiotic from Brevibacillus texasporus

Xiaofeng Wu, Johnathan Ballard, Yi Wei Jiang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We isolated a novel gram-positive bacterium, Brevibacillus texasporus, that produces an antibiotic, BT. BT is a group of related peptides that are produced by B. texasporus cells in response to nutrient limitation. We report here purification and determination of the structure of the most abundant BT isomer, BT1583. Amino acid composition and tandem mass spectrometry experiments yielded a partial BT1583 structure. The presence of ornithine and o-form residues in the partial BT1583 structure indicated that the peptide is synthesized by a nonribosomal peptide synthetase (NRPS). The BT NRPS operon was rapidly and accurately identified by using a novel in silico NRPS operon hunting strategy that involved direct shotgun genomic sequencing rather than the unreliable cosmid library hybridization scheme. Sequence analysis of the BT NRPS operon indicated that it encodes a colinear modular NRPS with a strict correlation between the NRPS modules and the amino acid residues in the peptide. The colinear nature of the BT NRPS enabled us to utilize the genomic information to refine the BT1583 peptide sequence to Me2-4-methyl-4-[(E)-2-butenyl]- 4,N-methyl-threonine-L-dO-I-V-V-dK-V-dL-K- dY-L-V-CH2OH. In addition, we report the discovery of novel NRPS codons (sets of the substrate specificity-conferring residues in NRPS modules) for valine, lysine, ornithine, and tyrosine.

Original languageEnglish (US)
Pages (from-to)8519-8530
Number of pages12
JournalApplied and Environmental Microbiology
Volume71
Issue number12
DOIs
StatePublished - Dec 1 2005

Fingerprint

Brevibacillus
peptide antibiotics
Peptide Synthases
antibiotics
peptide
biosynthesis
peptides
ligases
Operon
Ornithine
operon
Peptides
ornithine
Brevibacillus texasporus BT peptide antibiotic
Amino Acids
Cosmids
genomics
Firearms
Valine
Gram-Positive Bacteria

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Structure and biosynthesis of the BT peptide antibiotic from Brevibacillus texasporus. / Wu, Xiaofeng; Ballard, Johnathan; Jiang, Yi Wei.

In: Applied and Environmental Microbiology, Vol. 71, No. 12, 01.12.2005, p. 8519-8530.

Research output: Contribution to journalArticle

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