Structure and biosynthesis of the signal-sequence receptor

S. Prehn, J. Herz, E. Hartmann, T. V. Kurzchalia, R. Frank, K. Roemisch, B. Dobberstein, T. A. Rapoport

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The signal-sequence receptor (SSR) has previously been shown to be a component of the environment which nascent polypeptides meet on passage through the endoplasmic reticulum (ER) membrane. We report here on the primary structure of the SSR as deduced from cDNA clones and from direct protein sequencing. The glycoprotein is synthesized with a cleavable amino-terminal signal sequence and contains only one classical membrane-spanning segment. Its insertion into the ER membrane during biosynthesis depends on the function of the signal-recognition particle. SSR shows a remarkable charge distribution with the amino terminus being highly negatively charged, and the cytoplasmic carboxyl terminus positively charged. The SSR can be phosphorylated in its cytoplasmic tail both in intact cells and in a cell-free system, suggesting a regulation of its function. The localization of the protein in the protein in the ER membrane was confirmed by immunofluorescence microscopy.

Original languageEnglish (US)
Pages (from-to)439-445
Number of pages7
JournalEuropean Journal of Biochemistry
Volume188
Issue number2
StatePublished - 1990

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Biosynthesis
Endoplasmic Reticulum
Membranes
Signal Recognition Particle
Proteins
Cell-Free System
Charge distribution
Protein Sequence Analysis
Protein Sorting Signals
Fluorescence Microscopy
Glycoproteins
Microscopic examination
Complementary DNA
Clone Cells
Peptides
signal sequence receptor

ASJC Scopus subject areas

  • Biochemistry

Cite this

Prehn, S., Herz, J., Hartmann, E., Kurzchalia, T. V., Frank, R., Roemisch, K., ... Rapoport, T. A. (1990). Structure and biosynthesis of the signal-sequence receptor. European Journal of Biochemistry, 188(2), 439-445.

Structure and biosynthesis of the signal-sequence receptor. / Prehn, S.; Herz, J.; Hartmann, E.; Kurzchalia, T. V.; Frank, R.; Roemisch, K.; Dobberstein, B.; Rapoport, T. A.

In: European Journal of Biochemistry, Vol. 188, No. 2, 1990, p. 439-445.

Research output: Contribution to journalArticle

Prehn, S, Herz, J, Hartmann, E, Kurzchalia, TV, Frank, R, Roemisch, K, Dobberstein, B & Rapoport, TA 1990, 'Structure and biosynthesis of the signal-sequence receptor', European Journal of Biochemistry, vol. 188, no. 2, pp. 439-445.
Prehn S, Herz J, Hartmann E, Kurzchalia TV, Frank R, Roemisch K et al. Structure and biosynthesis of the signal-sequence receptor. European Journal of Biochemistry. 1990;188(2):439-445.
Prehn, S. ; Herz, J. ; Hartmann, E. ; Kurzchalia, T. V. ; Frank, R. ; Roemisch, K. ; Dobberstein, B. ; Rapoport, T. A. / Structure and biosynthesis of the signal-sequence receptor. In: European Journal of Biochemistry. 1990 ; Vol. 188, No. 2. pp. 439-445.
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AU - Dobberstein, B.

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