Structure and Ca2+-binding properties of the tandem C 2 Domains of E-Syt2

Junjie Xu, Taulant Bacaj, Amy Zhou, Diana R Tomchick, Thomas C. Südhof, Jose Rizo-Rey

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Contacts between the endoplasmic reticulum and the plasma membrane involve extended synaptotagmins (E-Syts) in mammals or tricalbins in yeast, proteins with multiple C2 domains. One of the tandem C2 domains of E-Syt2 is predicted to bind Ca2+, but no Ca2+-dependent function has been attributed to this protein. We have determined the crystal structures of the tandem C2 domains of E-Syt2 in the absence and presence of Ca2+ and analyzed their Ca2+-binding properties by nuclear magnetic resonance spectroscopy. Our data reveal an unexpected V-shaped structure with a rigid orientation between the two C 2 domains that is not substantially altered by Ca2+. The E-Syt2 C2A domain binds up to four Ca2+ ions, whereas the C2B domain does not bind Ca2+. These results suggest that E-Syt2 performs an as yet unidentified Ca2+-dependent function through its C2A domain and uncover fundamental differences between the properties of the tandem C2 domains of E-Syts and synaptotagmins.

Original languageEnglish (US)
Pages (from-to)269-280
Number of pages12
JournalStructure
Volume22
Issue number2
DOIs
StatePublished - Feb 4 2014

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Synaptotagmins
Fungal Proteins
Endoplasmic Reticulum
Mammals
Magnetic Resonance Spectroscopy
Cell Membrane
Ions
C2 Domains
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

Cite this

Structure and Ca2+-binding properties of the tandem C 2 Domains of E-Syt2. / Xu, Junjie; Bacaj, Taulant; Zhou, Amy; Tomchick, Diana R; Südhof, Thomas C.; Rizo-Rey, Jose.

In: Structure, Vol. 22, No. 2, 04.02.2014, p. 269-280.

Research output: Contribution to journalArticle

Xu, Junjie ; Bacaj, Taulant ; Zhou, Amy ; Tomchick, Diana R ; Südhof, Thomas C. ; Rizo-Rey, Jose. / Structure and Ca2+-binding properties of the tandem C 2 Domains of E-Syt2. In: Structure. 2014 ; Vol. 22, No. 2. pp. 269-280.
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