Structure and Expression of Murine Intestinal Trefoil Factor: High Evolutionary Conservation and Postnatal Expression

H. Mashimo, D. K. Podolsky, M. C. Fishman

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Intestinal Trefoil Factor (ITF) is a member of a family of gastrointestinal tract peptides with region-specific expression which are enhanced at sites of injury and repair. In the present study, the murine homologue gene of ITF was molecularly cloned in order to characterize the structure and expression of this peptide in mice. Murine ITF exhibited 78, 95 and 94% nucleotide homology respectively in exons I, II and III, with overall 90% predicted amino acid identity when compared to the rat ITF. Murine ITF exhibited 70% inferred amino acid identity compared with human ITF. Northern blot analysis of various adult mouse tissues demonstrated that ITF is expressed abundantly in the intestine and colon, and minimally in stomach, but not in brain, lung, spleen, kidney, uterus, pancreas, liver, heart or thymus tissues. Expression of ITF appeared to occur as a post-natal event: antibody specific for ITF stains intensely goblet cells in the intestine and colon of three-day old and older mice, but not in the gastrointestinal tract of younger mice or embryos.

Original languageEnglish (US)
Pages (from-to)31-37
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume210
Issue number1
DOIs
StatePublished - May 5 1995

Fingerprint

Conservation
Tissue
Amino Acids
Thymus
Peptides
Liver
Exons
Brain
Repair
Coloring Agents
Nucleotides
Genes
Antibodies
Intestines
Gastrointestinal Tract
Colon
Goblet Cells
Trefoil Factor-3
Northern Blotting
Thymus Gland

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Biophysics
  • Biochemistry

Cite this

Structure and Expression of Murine Intestinal Trefoil Factor : High Evolutionary Conservation and Postnatal Expression. / Mashimo, H.; Podolsky, D. K.; Fishman, M. C.

In: Biochemical and Biophysical Research Communications, Vol. 210, No. 1, 05.05.1995, p. 31-37.

Research output: Contribution to journalArticle

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