Structure and expression of the human L-myc gene reveal a complex pattern of alternative mRNA processing

F. Kaye, J. Battey, M. Nau, B. Brooks, E. Seifter, J. De Greve, M. Birrer, E. Sausville, J. Minna

Research output: Contribution to journalArticlepeer-review

70 Scopus citations

Abstract

We analyzed in detail the structure of the L-myc gene isolated from human placental DNA and characterized its expression in several small-cell lung cancer cell lines. The gene is composed of three exons and two introns spanning 6.6 kilobases in human DNA. Several distinct mRNA species are produced in all small-cell lung cancer cell lines that express L-myc. These transcripts are generated from a single gene by alternative splicing of introns 1 and 2 and by use of alternative polyadenylation signals. In some mRNAs there is a long open reading frame with a predicted translated protein of 364 residues. Amino acid sequence comparison with c-myc and N-myc demonstrated multiple discrete regions with extensive homology. In contrast, other mRNA transcripts, generated by alternative processing, could encode a truncated protein with a novel carboxy-terminal end.

Original languageEnglish (US)
Pages (from-to)186-195
Number of pages10
JournalMolecular and cellular biology
Volume8
Issue number1
DOIs
StatePublished - 1988

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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