Structure and specific RNA binding of ADAR2 double-stranded RNA binding motifs

Richard Stefl, Ming Xu, Lenka Skrisovska, Ronald B. Emeson, Frédéric H T Allain

Research output: Contribution to journalArticle

79 Scopus citations

Abstract

Adenosine deaminases that act on RNA (ADARs) site-selectively modify adenosines to inosines within RNA transcripts, thereby recoding genomic information. How ADARs select specific adenosine moieties for deamination is poorly understood. Here, we report NMR structures of the two double-stranded RNA binding motifs (dsRBMs) of rat ADAR2 and an NMR chemical shift perturbation study of the interaction of the two dsRBMs with a 71 nucleotide RNA encoding the R/G site of the GluR-B. We have identified the protein and the RNA surfaces involved in complex formation, allowing us to present an NMR-based model of the complex. We have found that dsRBM1 recognizes a conserved pentaloop, whereas dsRBM2 recognizes two bulged bases adjacent to the editing site, demonstrating RNA structure-dependent recognition by the ADAR2 dsRBMs. In vitro mutagenesis studies with both the protein and the RNA further support our structural findings.

Original languageEnglish (US)
Pages (from-to)345-355
Number of pages11
JournalStructure
Volume14
Issue number2
DOIs
StatePublished - Feb 1 2006

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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    Stefl, R., Xu, M., Skrisovska, L., Emeson, R. B., & Allain, F. H. T. (2006). Structure and specific RNA binding of ADAR2 double-stranded RNA binding motifs. Structure, 14(2), 345-355. https://doi.org/10.1016/j.str.2005.11.013