Structure-based Moloney murine leukemia virus reverse transcriptase mutants with altered intracellular direct-repeat deletion frequencies

J. K. Pfeiffer, M. M. Georgiadis, A. Telesnitsky

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Template switching rates of Moloney murine leukemia virus reverse transcriptase mutants were tested using a retroviral vector-based direct-repeat deletion assay. The reverse transcriptase mutants contained alterations in residues that modeling of substrates into the catalytic core had suggested might affect interactions with primer and/or template strands. As assessed by the frequency of functional lacZ gene generation from vectors in which lacZ was disrupted by insertion of a sequence duplication, the frequency of template switching varied more than threefold among fully replication-competent mutants. Some mutants displayed deletion rates that were lower and others displayed rates that were higher than that of wild-type virus. Replication for the mutants with the most significant alterations in template switching frequencies was similar to that of the wild type. These data suggest that reverse transcriptase template switching rates can be altered significantly without destroying normal replication functions.

Original languageEnglish (US)
Pages (from-to)9629-9636
Number of pages8
JournalJournal of Virology
Volume74
Issue number20
DOIs
StatePublished - 2000

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Murine leukemia virus
Moloney murine leukemia virus
RNA-directed DNA polymerase
Nucleic Acid Repetitive Sequences
RNA-Directed DNA Polymerase
mutants
Lac Operon
Insertional Mutagenesis
retroviral vectors
Catalytic Domain
Viruses
transposons
viruses
assays
genes

ASJC Scopus subject areas

  • Immunology

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Structure-based Moloney murine leukemia virus reverse transcriptase mutants with altered intracellular direct-repeat deletion frequencies. / Pfeiffer, J. K.; Georgiadis, M. M.; Telesnitsky, A.

In: Journal of Virology, Vol. 74, No. 20, 2000, p. 9629-9636.

Research output: Contribution to journalArticle

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