Structure Guided Design, Synthesis, and Biological Evaluation of Novel Benzosuberene Analogues as Inhibitors of Tubulin Polymerization

Haichan Niu, Tracy E. Strecker, Jeni L. Gerberich, James W. Campbell, Debabrata Saha, Deboprosad Mondal, Ernest Hamel, David J. Chaplin, Ralph P Mason, Mary Lynn Trawick, Kevin G. Pinney

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A promising design paradigm for small-molecule inhibitors of tubulin polymerization that bind to the colchicine site draws structural inspiration from the natural products colchicine and combretastatin A-4 (CA4). Our previous studies with benzocycloalkenyl and heteroaromatic ring systems yielded promising inhibitors with dihydronaphthalene and benzosuberene analogues featuring phenolic (KGP03 and KGP18) and aniline (KGP05 and KGP156) congeners emerging as lead agents. These molecules demonstrated dual mechanism of action, functioning both as potent vascular disrupting agents (VDAs) and as highly cytotoxic anticancer agents. A further series of analogues was designed to extend functional group diversity and investigate regioisomeric tolerance. Ten new molecules were effective inhibitors of tubulin polymerization (IC50 < 5 μM) with seven of these exhibiting highly potent activity comparable to CA4, KGP18, and KGP03. For one of the most effective agents, dose-dependent vascular shutdown was demonstrated using dynamic bioluminescence imaging in a human prostate tumor xenograft growing in a rat.

Original languageEnglish (US)
Pages (from-to)5594-5615
Number of pages22
JournalJournal of Medicinal Chemistry
Volume62
Issue number11
DOIs
StatePublished - Jun 13 2019

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Tubulin Modulators
Colchicine
Blood Vessels
Cytotoxins
Biological Products
Heterografts
Antineoplastic Agents
Inhibitory Concentration 50
Prostate
Neoplasms
fosbretabulin
benzosuberene

ASJC Scopus subject areas

  • Molecular Medicine
  • Drug Discovery

Cite this

Structure Guided Design, Synthesis, and Biological Evaluation of Novel Benzosuberene Analogues as Inhibitors of Tubulin Polymerization. / Niu, Haichan; Strecker, Tracy E.; Gerberich, Jeni L.; Campbell, James W.; Saha, Debabrata; Mondal, Deboprosad; Hamel, Ernest; Chaplin, David J.; Mason, Ralph P; Trawick, Mary Lynn; Pinney, Kevin G.

In: Journal of Medicinal Chemistry, Vol. 62, No. 11, 13.06.2019, p. 5594-5615.

Research output: Contribution to journalArticle

Niu, H, Strecker, TE, Gerberich, JL, Campbell, JW, Saha, D, Mondal, D, Hamel, E, Chaplin, DJ, Mason, RP, Trawick, ML & Pinney, KG 2019, 'Structure Guided Design, Synthesis, and Biological Evaluation of Novel Benzosuberene Analogues as Inhibitors of Tubulin Polymerization', Journal of Medicinal Chemistry, vol. 62, no. 11, pp. 5594-5615. https://doi.org/10.1021/acs.jmedchem.9b00551
Niu, Haichan ; Strecker, Tracy E. ; Gerberich, Jeni L. ; Campbell, James W. ; Saha, Debabrata ; Mondal, Deboprosad ; Hamel, Ernest ; Chaplin, David J. ; Mason, Ralph P ; Trawick, Mary Lynn ; Pinney, Kevin G. / Structure Guided Design, Synthesis, and Biological Evaluation of Novel Benzosuberene Analogues as Inhibitors of Tubulin Polymerization. In: Journal of Medicinal Chemistry. 2019 ; Vol. 62, No. 11. pp. 5594-5615.
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