Structure of a fucose transporter in an outward-open conformation

Shangyu Dang, Linfeng Sun, Yongjian Huang, Feiran Lu, Yufeng Liu, Haipeng Gong, Jiawei Wang, Nieng Yan

Research output: Contribution to journalArticle

186 Citations (Scopus)

Abstract

The major facilitator superfamily (MFS) transporters are an ancient and widespread family of secondary active transporters. In Escherichia coli, the uptake of l-fucose, a source of carbon for microorganisms, is mediated by an MFS proton symporter, FucP. Despite intensive study of the MFS transporters, atomic structure information is only available on three proteins and the outward-open conformation has yet to be captured. Here we report the crystal structure of FucP at 3.1 Å resolution, which shows that it contains an outward-open, amphipathic cavity. The similarly folded amino and carboxyl domains of FucP have contrasting surface features along the transport path, with negative electrostatic potential on the N domain and hydrophobic surface on the C domain. FucP only contains two acidic residues along the transport path, Asp 46 and Glu 135, which can undergo cycles of protonation and deprotonation. Their essential role in active transport is supported by both in vivo and in vitro experiments. Structure-based biochemical analyses provide insights into energy coupling, substrate recognition and the transport mechanism of FucP.

Original languageEnglish (US)
Pages (from-to)734-738
Number of pages5
JournalNature
Volume467
Issue number7316
DOIs
StatePublished - Oct 7 2010

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Symporters
Fucose
Active Biological Transport
Static Electricity
Protons
Carbon
Escherichia coli
Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • Medicine(all)
  • General

Cite this

Dang, S., Sun, L., Huang, Y., Lu, F., Liu, Y., Gong, H., ... Yan, N. (2010). Structure of a fucose transporter in an outward-open conformation. Nature, 467(7316), 734-738. https://doi.org/10.1038/nature09406

Structure of a fucose transporter in an outward-open conformation. / Dang, Shangyu; Sun, Linfeng; Huang, Yongjian; Lu, Feiran; Liu, Yufeng; Gong, Haipeng; Wang, Jiawei; Yan, Nieng.

In: Nature, Vol. 467, No. 7316, 07.10.2010, p. 734-738.

Research output: Contribution to journalArticle

Dang, S, Sun, L, Huang, Y, Lu, F, Liu, Y, Gong, H, Wang, J & Yan, N 2010, 'Structure of a fucose transporter in an outward-open conformation', Nature, vol. 467, no. 7316, pp. 734-738. https://doi.org/10.1038/nature09406
Dang S, Sun L, Huang Y, Lu F, Liu Y, Gong H et al. Structure of a fucose transporter in an outward-open conformation. Nature. 2010 Oct 7;467(7316):734-738. https://doi.org/10.1038/nature09406
Dang, Shangyu ; Sun, Linfeng ; Huang, Yongjian ; Lu, Feiran ; Liu, Yufeng ; Gong, Haipeng ; Wang, Jiawei ; Yan, Nieng. / Structure of a fucose transporter in an outward-open conformation. In: Nature. 2010 ; Vol. 467, No. 7316. pp. 734-738.
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