Structure of a human synaptic GABAA receptor

Shaotong Zhu, Colleen M. Noviello, Jinfeng Teng, Richard M. Walsh, Jeong Joo Kim, Ryan E Hibbs

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Fast inhibitory neurotransmission in the brain is principally mediated by the neurotransmitter GABA (γ-aminobutyric acid) and its synaptic target, the type A GABA receptor (GABAA receptor). Dysfunction of this receptor results in neurological disorders and mental illnesses including epilepsy, anxiety and insomnia. The GABAA receptor is also a prolific target for therapeutic, illicit and recreational drugs, including benzodiazepines, barbiturates, anaesthetics and ethanol. Here we present high-resolution cryo-electron microscopy structures of the human α1β2γ2 GABAA receptor, the predominant isoform in the adult brain, in complex with GABA and the benzodiazepine site antagonist flumazenil, the first-line clinical treatment for benzodiazepine overdose. The receptor architecture reveals unique heteromeric interactions for this important class of inhibitory neurotransmitter receptor. This work provides a template for understanding receptor modulation by GABA and benzodiazepines, and will assist rational approaches to therapeutic targeting of this receptor for neurological disorders and mental illness.

Original languageEnglish (US)
Pages (from-to)67-88
Number of pages22
JournalNature
Volume559
Issue number7712
DOIs
StatePublished - Jan 1 2018

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Neurotransmitter Receptor
GABA-A Receptors
Benzodiazepines
Street Drugs
Nervous System Diseases
gamma-Aminobutyric Acid
Aminobutyrates
Cryoelectron Microscopy
Flumazenil
Barbiturates
Brain
Sleep Initiation and Maintenance Disorders
Synaptic Transmission
Neurotransmitter Agents
Anesthetics
Epilepsy
Protein Isoforms
Ethanol
Anxiety
Therapeutics

ASJC Scopus subject areas

  • General

Cite this

Zhu, S., Noviello, C. M., Teng, J., Walsh, R. M., Kim, J. J., & Hibbs, R. E. (2018). Structure of a human synaptic GABAA receptor. Nature, 559(7712), 67-88. https://doi.org/10.1038/s41586-018-0255-3

Structure of a human synaptic GABAA receptor. / Zhu, Shaotong; Noviello, Colleen M.; Teng, Jinfeng; Walsh, Richard M.; Kim, Jeong Joo; Hibbs, Ryan E.

In: Nature, Vol. 559, No. 7712, 01.01.2018, p. 67-88.

Research output: Contribution to journalArticle

Zhu, S, Noviello, CM, Teng, J, Walsh, RM, Kim, JJ & Hibbs, RE 2018, 'Structure of a human synaptic GABAA receptor', Nature, vol. 559, no. 7712, pp. 67-88. https://doi.org/10.1038/s41586-018-0255-3
Zhu S, Noviello CM, Teng J, Walsh RM, Kim JJ, Hibbs RE. Structure of a human synaptic GABAA receptor. Nature. 2018 Jan 1;559(7712):67-88. https://doi.org/10.1038/s41586-018-0255-3
Zhu, Shaotong ; Noviello, Colleen M. ; Teng, Jinfeng ; Walsh, Richard M. ; Kim, Jeong Joo ; Hibbs, Ryan E. / Structure of a human synaptic GABAA receptor. In: Nature. 2018 ; Vol. 559, No. 7712. pp. 67-88.
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