Structure of an RNA polymerase II-TFIIB complex and the transcription initiation mechanism

Xin Liu, David A. Bushnell, Dong Wang, Guillermo Calero, Roger D. Kornbergt

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

Previous x-ray crystal structures have given insight into the mechanism of transcription and the role of general transcription factors in the initiation of the process. A structure of an RNA polymerase II-general transcription factor TFIIB complex at 4.5 angstrom resolution revealed the amino-terminal region of TFIIB, including a loop termed the "B finger," reaching into the active center of the polymerase where it may interact with both DNA and RNA, but this structure showed little of the carboxyl-terminal region. A new crystal structure of the same complex at 3.8 angstrom resolution obtained under different solution conditions is complementary with the previous one, revealing the carboxyl-terminal region of TFIIB, located above the polymerase active center cleft, but showing none of the B finger. In the new structure, the linker between the amino- and carboxyl-terminal regions can also be seen, snaking down from above the cleft toward the active center. The two structures, taken together with others previously obtained, dispel long-standing mysteries of the transcription initiation process.

Original languageEnglish (US)
Pages (from-to)206-209
Number of pages4
JournalScience
Volume327
Issue number5962
DOIs
StatePublished - 2010

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Transcription Factor TFIIB
RNA Polymerase II
General Transcription Factors
Fingers
X-Rays
RNA
DNA

ASJC Scopus subject areas

  • General

Cite this

Structure of an RNA polymerase II-TFIIB complex and the transcription initiation mechanism. / Liu, Xin; Bushnell, David A.; Wang, Dong; Calero, Guillermo; Kornbergt, Roger D.

In: Science, Vol. 327, No. 5962, 2010, p. 206-209.

Research output: Contribution to journalArticle

Liu, X, Bushnell, DA, Wang, D, Calero, G & Kornbergt, RD 2010, 'Structure of an RNA polymerase II-TFIIB complex and the transcription initiation mechanism', Science, vol. 327, no. 5962, pp. 206-209. https://doi.org/10.1126/science.1182015
Liu, Xin ; Bushnell, David A. ; Wang, Dong ; Calero, Guillermo ; Kornbergt, Roger D. / Structure of an RNA polymerase II-TFIIB complex and the transcription initiation mechanism. In: Science. 2010 ; Vol. 327, No. 5962. pp. 206-209.
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