Structure of cortical network activity across natural wake and sleep states in mice

Kaoru Ohyama, Takeshi Kanda, Takehiro Miyazaki, Natsuko Tsujino, Ryo Ishii, Yukiko Ishikawa, Hiroki Muramoto, Francois Grenier, Yuichi Makino, Thomas J. McHugh, Masashi Yanagisawa, Robert W. Greene, Kaspar E. Vogt

Research output: Contribution to journalArticle

Abstract

Cortical neurons fire intermittently and synchronously during non-rapid eye movement sleep (NREMS), in which active and silent periods are referred to as ON and OFF periods, respectively. Neuronal firing rates during ON periods (NREMS-ON-Activity) are similar to those of wakefulness (W-Activity), raising the possibility that NREMS-ON neuronal-Activity is fragmented W-Activity. To test this, we investigated the patterning and organization of cortical spike trains and of spike ensembles in neuronal networks using extracellular recordings in mice. Firing rates of neurons during NREMS-ON andWwere similar, but showed enhanced bursting in NREMS with no apparent preference in occurrence, relative to the beginning or end of the on-state. Additionally, there was an overall increase in the randomness of occurrence of sequences comprised of multi-neuron ensembles in NREMS recorded from tetrodes. In association with increased burst firing, somatic calcium transients were increased in NREMS. The increased calcium transients associated with bursting during NREM may activate calcium-dependent, cell-signaling pathways for sleep related cellular processes.

Original languageEnglish (US)
Article numbere0233561
JournalPloS one
Volume15
Issue number5
DOIs
StatePublished - May 2020

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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  • Cite this

    Ohyama, K., Kanda, T., Miyazaki, T., Tsujino, N., Ishii, R., Ishikawa, Y., Muramoto, H., Grenier, F., Makino, Y., McHugh, T. J., Yanagisawa, M., Greene, R. W., & Vogt, K. E. (2020). Structure of cortical network activity across natural wake and sleep states in mice. PloS one, 15(5), [e0233561]. https://doi.org/10.1371/journal.pone.0233561