TY - JOUR
T1 - Structure of the gene encoding CD34, a human hematopoietic stem cell antigen
AU - Satterthwaite, Anne B.
AU - Burn, Timothy C.
AU - Le Beau, Michelle M.
AU - Tenen, Daniel G.
N1 - Funding Information:
The authors thank David Simmons and Brian Seed for providing the CD34 cDNA and Rachel Borson for isolating the clones Lambda-RB and CD348 We also acknowledge Rafael Espinosa III and Yo-gesh Pate1 for technical assistance, Helene F. Rosenberg, Steven J. Ackerman, and Linda K. Clayton for criticism of the manuscript, and Judith E. Coffin for helpful discussions. This work was supported by NIH Grant CA34183. D.G.T. is a scholar of the Leukemia Society of America.
PY - 1992/4
Y1 - 1992/4
N2 - CD34 is a cell surface antigen of unknown function expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30% of patients with acute myeloid and lymphocytic leukemia. To begin to investigate the cis-acting elements required for this tissue-specific expression, the human CD34 locus was isolated and its genomic structure and transcriptional start site were characterized. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. The start site of CD34 transcription was determined to be 258 bp upstream of the translational start site using RNase protection. These experiments also indicated that the 5′ untranslated region has extensive secondary structure. In addition, fluorescence in situ hybridization was used to map the CD34 locus to band 1q32.
AB - CD34 is a cell surface antigen of unknown function expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30% of patients with acute myeloid and lymphocytic leukemia. To begin to investigate the cis-acting elements required for this tissue-specific expression, the human CD34 locus was isolated and its genomic structure and transcriptional start site were characterized. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. The start site of CD34 transcription was determined to be 258 bp upstream of the translational start site using RNase protection. These experiments also indicated that the 5′ untranslated region has extensive secondary structure. In addition, fluorescence in situ hybridization was used to map the CD34 locus to band 1q32.
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U2 - 10.1016/0888-7543(92)90310-O
DO - 10.1016/0888-7543(92)90310-O
M3 - Article
C2 - 1374051
AN - SCOPUS:0026517799
SN - 0888-7543
VL - 12
SP - 788
EP - 794
JO - Genomics
JF - Genomics
IS - 4
ER -