Structure of the gene encoding CD34, a human hematopoietic stem cell antigen

Anne B. Satterthwaite, Timothy C. Burn, Michelle M. Le Beau, Daniel G. Tenen

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

CD34 is a cell surface antigen of unknown function expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30% of patients with acute myeloid and lymphocytic leukemia. To begin to investigate the cis-acting elements required for this tissue-specific expression, the human CD34 locus was isolated and its genomic structure and transcriptional start site were characterized. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. The start site of CD34 transcription was determined to be 258 bp upstream of the translational start site using RNase protection. These experiments also indicated that the 5′ untranslated region has extensive secondary structure. In addition, fluorescence in situ hybridization was used to map the CD34 locus to band 1q32.

Original languageEnglish (US)
Pages (from-to)788-794
Number of pages7
JournalGenomics
Volume12
Issue number4
DOIs
StatePublished - 1992

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Hematopoietic Stem Cells
Antigens
Genes
5' Untranslated Regions
Transcription Initiation Site
Vascular Endothelium
Surface Antigens
Ribonucleases
Fluorescence In Situ Hybridization
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Acute Myeloid Leukemia
Exons

ASJC Scopus subject areas

  • Genetics

Cite this

Structure of the gene encoding CD34, a human hematopoietic stem cell antigen. / Satterthwaite, Anne B.; Burn, Timothy C.; Le Beau, Michelle M.; Tenen, Daniel G.

In: Genomics, Vol. 12, No. 4, 1992, p. 788-794.

Research output: Contribution to journalArticle

Satterthwaite, Anne B. ; Burn, Timothy C. ; Le Beau, Michelle M. ; Tenen, Daniel G. / Structure of the gene encoding CD34, a human hematopoietic stem cell antigen. In: Genomics. 1992 ; Vol. 12, No. 4. pp. 788-794.
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