C2 domains are widespread protein modules that often occur as tandem repeats in many membrane-trafficking proteins such as synaptotagmin and rabphilin. The first and second C2 domains (C2A and C2B, respectively) have a high degree of homology but also specific differences. The structure of the C2A domain of synaptotagmin I has been extensively studied but little is known about the C2B domains. We have used NMR spectroscopy to determine the solution structure of the C2B domain of rabphilin. The overall structure of the C2B domain is very similar to that of other C2 domains, with a rigid β-sandwich core and loops at the top (where Ca2+ binds) and the bottom. Surprisingly, a relatively long α-helix is inserted at the bottom of the domain and is conserved in all C2B domains. Our results, together with the Ca2+-independent interactions observed for C2B domains, indicate that these domains have a Janus-faced nature, with a Ca2+-binding top surface and a Ca2+-independent bottom surface.
|Original language||English (US)|
|Number of pages||7|
|Journal||Nature Cell Biology|
|Publication status||Published - Jun 1999|
ASJC Scopus subject areas
- Cell Biology