Structure of the Janus-faced C2B domain of rabphilin

Josep Ubach, Jesús García, M. Paige Nittler, Thomas C. Südhof, Jose Rizo-Rey

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

C2 domains are widespread protein modules that often occur as tandem repeats in many membrane-trafficking proteins such as synaptotagmin and rabphilin. The first and second C2 domains (C2A and C2B, respectively) have a high degree of homology but also specific differences. The structure of the C2A domain of synaptotagmin I has been extensively studied but little is known about the C2B domains. We have used NMR spectroscopy to determine the solution structure of the C2B domain of rabphilin. The overall structure of the C2B domain is very similar to that of other C2 domains, with a rigid β-sandwich core and loops at the top (where Ca2+ binds) and the bottom. Surprisingly, a relatively long α-helix is inserted at the bottom of the domain and is conserved in all C2B domains. Our results, together with the Ca2+-independent interactions observed for C2B domains, indicate that these domains have a Janus-faced nature, with a Ca2+-binding top surface and a Ca2+-independent bottom surface.

Original languageEnglish (US)
Pages (from-to)106-112
Number of pages7
JournalNature Cell Biology
Volume1
Issue number2
StatePublished - Jun 1999

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Synaptotagmin I
Synaptotagmins
Tandem Repeat Sequences
Membrane Proteins
Magnetic Resonance Spectroscopy
C2 Domains
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Ubach, J., García, J., Nittler, M. P., Südhof, T. C., & Rizo-Rey, J. (1999). Structure of the Janus-faced C2B domain of rabphilin. Nature Cell Biology, 1(2), 106-112.

Structure of the Janus-faced C2B domain of rabphilin. / Ubach, Josep; García, Jesús; Nittler, M. Paige; Südhof, Thomas C.; Rizo-Rey, Jose.

In: Nature Cell Biology, Vol. 1, No. 2, 06.1999, p. 106-112.

Research output: Contribution to journalArticle

Ubach, J, García, J, Nittler, MP, Südhof, TC & Rizo-Rey, J 1999, 'Structure of the Janus-faced C2B domain of rabphilin', Nature Cell Biology, vol. 1, no. 2, pp. 106-112.
Ubach J, García J, Nittler MP, Südhof TC, Rizo-Rey J. Structure of the Janus-faced C2B domain of rabphilin. Nature Cell Biology. 1999 Jun;1(2):106-112.
Ubach, Josep ; García, Jesús ; Nittler, M. Paige ; Südhof, Thomas C. ; Rizo-Rey, Jose. / Structure of the Janus-faced C2B domain of rabphilin. In: Nature Cell Biology. 1999 ; Vol. 1, No. 2. pp. 106-112.
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