Structure of the mouse klotho gene and its two transcripts encoding membrane and secreted protein

Takako Shiraki-Iida, Hiroki Aizawa, Yutaka Matsumura, Susumu Sekine, Akihiro Iida, Hideharu Anazawa, Ryozo Nagai, Makoto Kuro-O, Yo Ichi Nabeshima

Research output: Contribution to journalArticle

208 Scopus citations

Abstract

We previously established a novel mouse model for human aging and identified the genetic foundation responsible for it. A defect in expression of a novel gene, termed klotho (kl), leads to a syndrome resembling human aging in mice. The kl gene encodes a single-pass membrane protein whose extracellular domain carries homology to β-glucosidases. In this report, we present the entire mouse kl gene organization. The mouse kl gene spans about 50 kilobases and consists of five exons. The promoter region lacks a TATA-box and contains four potential binding sites for SP1. We further show that two kl gene transcripts encoding membrane or secreted protein are generated through alternative transcriptional termination. These findings provide fundamental information for further study of the kl gene which may regulate aging in vivo.

Original languageEnglish (US)
Pages (from-to)6-10
Number of pages5
JournalFEBS Letters
Volume424
Issue number1-2
DOIs
StatePublished - Mar 6 1998

Keywords

  • Alternative transcriptional termination
  • Competitive polymerase chain reaction
  • Mouse kotho gene

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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    Shiraki-Iida, T., Aizawa, H., Matsumura, Y., Sekine, S., Iida, A., Anazawa, H., Nagai, R., Kuro-O, M., & Nabeshima, Y. I. (1998). Structure of the mouse klotho gene and its two transcripts encoding membrane and secreted protein. FEBS Letters, 424(1-2), 6-10. https://doi.org/10.1016/S0014-5793(98)00127-6