Structure of the rat gene encoding cholesterol 7α-hydroxylase

Diane F. Jelinek, David W. Russell

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Cholesterol 7α-hydroxylase (7α-hydroxylase) is a microsomal cytochrome P-450 that catalyzes the first and rate-limiting step in bile acid biosynthesis, the major catabolic pathway in cholesterol homeostasis. The gene encoding the rat 7α-hydroxylase has been isolated and characterized. Southern blotting experiments demonstrated that the gene is present in a single copy in the rat genome. DNA sequence analysis showed that the 7α-hydroxylase gene is unique among the characterized cytochrome P-450s in that it contains only six exons. Nuclease S1 and primer-extension mapping experiments positioned the 5′-ends of the 7α-hydroxylase mRNA approximately 20-25 nucleotides downstream of a consensus TATAAA sequence. RNA blotting experiments demonstrated the presence of multiple 7α-hydroxylase mRNAs that differ in the lengths of their 3′-untranslated regions.

Original languageEnglish (US)
Pages (from-to)7781-7785
Number of pages5
JournalBiochemistry
Volume29
Issue number34
StatePublished - 1990

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Cholesterol 7-alpha-Hydroxylase
Gene encoding
Mixed Function Oxygenases
Rats
Genes
Messenger RNA
Experiments
DNA sequences
Biosynthesis
Consensus Sequence
3' Untranslated Regions
Cytochromes
Southern Blotting
Bile Acids and Salts
DNA Sequence Analysis
Cytochrome P-450 Enzyme System
Exons
Homeostasis
Nucleotides
Cholesterol

ASJC Scopus subject areas

  • Biochemistry

Cite this

Structure of the rat gene encoding cholesterol 7α-hydroxylase. / Jelinek, Diane F.; Russell, David W.

In: Biochemistry, Vol. 29, No. 34, 1990, p. 7781-7785.

Research output: Contribution to journalArticle

Jelinek, Diane F. ; Russell, David W. / Structure of the rat gene encoding cholesterol 7α-hydroxylase. In: Biochemistry. 1990 ; Vol. 29, No. 34. pp. 7781-7785.
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