Structure of the Type III Secretion Effector Protein ExoU in Complex with Its Chaperone SpcU

Andrei S. Halavaty, Dominika Borek, Gregory H. Tyson, Jeff L. Veesenmeyer, Ludmilla Shuvalova, George Minasov, Zbyszek Otwinowski, Alan R. Hauser, Wayne F. Anderson

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Disease causing bacteria often manipulate host cells in a way that facilitates the infectious process. Many pathogenic gram-negative bacteria accomplish this by using type III secretion systems. In these complex secretion pathways, bacterial chaperones direct effector proteins to a needle-like secretion apparatus, which then delivers the effector protein into the host cell cytosol. The effector protein ExoU and its chaperone SpcU are components of the Pseudomonas aeruginosa type III secretion system. Secretion of ExoU has been associated with more severe infections in both humans and animal models. Here we describe the 1.92 Å X-ray structure of the ExoU-SpcU complex, a full-length type III effector in complex with its full-length cognate chaperone. Our crystallographic data allow a better understanding of the mechanism by which ExoU kills host cells and provides a foundation for future studies aimed at designing inhibitors of this potent toxin.

Original languageEnglish (US)
Article numbere49388
JournalPLoS One
Volume7
Issue number11
DOIs
StatePublished - Nov 14 2012

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type III secretion system
secretion
Bacteria
Proteins
proteins
Secretory Pathway
cells
Gram-Negative Bacteria
Pseudomonas aeruginosa
Gram-negative bacteria
cytosol
Needles
Cytosol
Animals
X-radiation
toxins
Animal Models
animal models
X-Rays
X rays

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Halavaty, A. S., Borek, D., Tyson, G. H., Veesenmeyer, J. L., Shuvalova, L., Minasov, G., ... Anderson, W. F. (2012). Structure of the Type III Secretion Effector Protein ExoU in Complex with Its Chaperone SpcU. PLoS One, 7(11), [e49388]. https://doi.org/10.1371/journal.pone.0049388

Structure of the Type III Secretion Effector Protein ExoU in Complex with Its Chaperone SpcU. / Halavaty, Andrei S.; Borek, Dominika; Tyson, Gregory H.; Veesenmeyer, Jeff L.; Shuvalova, Ludmilla; Minasov, George; Otwinowski, Zbyszek; Hauser, Alan R.; Anderson, Wayne F.

In: PLoS One, Vol. 7, No. 11, e49388, 14.11.2012.

Research output: Contribution to journalArticle

Halavaty, AS, Borek, D, Tyson, GH, Veesenmeyer, JL, Shuvalova, L, Minasov, G, Otwinowski, Z, Hauser, AR & Anderson, WF 2012, 'Structure of the Type III Secretion Effector Protein ExoU in Complex with Its Chaperone SpcU', PLoS One, vol. 7, no. 11, e49388. https://doi.org/10.1371/journal.pone.0049388
Halavaty, Andrei S. ; Borek, Dominika ; Tyson, Gregory H. ; Veesenmeyer, Jeff L. ; Shuvalova, Ludmilla ; Minasov, George ; Otwinowski, Zbyszek ; Hauser, Alan R. ; Anderson, Wayne F. / Structure of the Type III Secretion Effector Protein ExoU in Complex with Its Chaperone SpcU. In: PLoS One. 2012 ; Vol. 7, No. 11.
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