Structure of the yeast mitochondrial

Alexey Amunts, Alan Brown, Xiao Chen Bai, Jose L. Llácer, Tanweer Hussain, Paul Emsley, Fei Long, Garib Murshudov, Sjors H.W. Scheres, V. Ramakrishnan

Research output: Contribution to journalArticle

306 Scopus citations

Abstract

Mitochondria have specialized ribosomes that have diverged from their bacterial and cytoplasmic counterparts.We have solved the structure of the yeast mitoribosomal large subunit using single-particle cryo-electron microscopy. The resolution of 3.2 angstroms enabled a nearly complete atomic model to be built de novo and refined, including 39 proteins, 13 of which are unique to mitochondria, as well as expansion segments of mitoribosomal RNA. The structure reveals a new exit tunnel path and architecture, unique elements of the E site, and a putative membrane docking site.

Original languageEnglish (US)
Pages (from-to)1485-1489
Number of pages5
JournalScience
Volume343
Issue number6178
DOIs
StatePublished - Jan 1 2014

ASJC Scopus subject areas

  • General

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    Amunts, A., Brown, A., Bai, X. C., Llácer, J. L., Hussain, T., Emsley, P., Long, F., Murshudov, G., Scheres, S. H. W., & Ramakrishnan, V. (2014). Structure of the yeast mitochondrial. Science, 343(6178), 1485-1489. https://doi.org/10.1126/science.1249410