Abstract
In wide-field fluorescence microscopy, illuminating the specimen with evanescent standing waves increases lateral resolution more than twofold. We report a versatile setup for standing-wave illumination in total internal reflection fluorescence microscopy. An adjustable diffraction grating written on a phase-only spatial light modulator controls the illumination field. Selecting appropriate diffraction orders and displaying a sheared (tilted) diffraction grating allows one to tune the penetration depth in very fine steps. The setup achieves 91 nm lateral resolution for green emission.
Original language | English (US) |
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Pages (from-to) | 1629-1631 |
Number of pages | 3 |
Journal | Optics Letters |
Volume | 33 |
Issue number | 14 |
DOIs | |
State | Published - Jul 15 2008 |
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics