Structured illumination in total internal reflection fluorescence microscopy using a spatial light modulator

Reto Fiolka, Markus Beck, Andreas Stemmer

Research output: Contribution to journalArticle

73 Scopus citations

Abstract

In wide-field fluorescence microscopy, illuminating the specimen with evanescent standing waves increases lateral resolution more than twofold. We report a versatile setup for standing-wave illumination in total internal reflection fluorescence microscopy. An adjustable diffraction grating written on a phase-only spatial light modulator controls the illumination field. Selecting appropriate diffraction orders and displaying a sheared (tilted) diffraction grating allows one to tune the penetration depth in very fine steps. The setup achieves 91 nm lateral resolution for green emission.

Original languageEnglish (US)
Pages (from-to)1629-1631
Number of pages3
JournalOptics Letters
Volume33
Issue number14
DOIs
StatePublished - Jul 15 2008

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ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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