Structures of the human mitochondrial ribosome in native states of assembly

Alan Brown, Sorbhi Rathore, Dari Kimanius, Shintaro Aibara, Xiao Chen Bai, Joanna Rorbach, Alexey Amunts, V. Ramakrishnan

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

Mammalian mitochondrial ribosomes (mitoribosomes) have less rRNA content and 36 additional proteins compared with the evolutionarily related bacterial ribosome. These differences make the assembly of mitoribosomes more complex than the assembly of bacterial ribosomes, but the molecular details of mitoribosomal biogenesis remain elusive. Here, we report the structures of two late-stage assembly intermediates of the human mitoribosomal large subunit (mt-LSU) isolated from a native pool within a human cell line and solved by cryo-EM to 3-resolution. Comparison of the structures reveals insights into the timing of rRNA folding and protein incorporation during the final steps of ribosomal maturation and the evolutionary adaptations that are required to preserve biogenesis after the structural diversification of mitoribosomes. Furthermore, the structures redefine the ribosome silencing factor (RsfS) family as multifunctional biogenesis factors and identify two new assembly factors (L0R8F8 and mt-ACP) not previously implicated in mitoribosomal biogenesis.

Original languageEnglish (US)
Pages (from-to)866-869
Number of pages4
JournalNature Structural and Molecular Biology
Volume24
Issue number10
DOIs
StatePublished - Oct 5 2017

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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    Brown, A., Rathore, S., Kimanius, D., Aibara, S., Bai, X. C., Rorbach, J., Amunts, A., & Ramakrishnan, V. (2017). Structures of the human mitochondrial ribosome in native states of assembly. Nature Structural and Molecular Biology, 24(10), 866-869. https://doi.org/10.1038/nsmb.3464