TY - JOUR
T1 - Studies on cell communication with enucleated human fibroblasts
AU - Cox, R. P.
AU - Krauss, M. R.
AU - Balis, M. E.
AU - Dancis, J.
PY - 1976/12/1
Y1 - 1976/12/1
N2 - Metabolic cooperation, the correction of the mutant phenotype in cells deficient in hypoxanthine phosphoribosyltransferase (HPRT−) by intimate contact with normal cells (HPRT+), represents a form of cell commimication that is easily studied with radioautography. In the present study it was found that the formation of cell junctions needed for communication does not require protein synthesis nor is it under the immediate control of the cell nucleus. Enucleated normal cells efficiently communicate with HPRT− mutant cells. The effectiveness of enucleated cells as donors in metabolic cooperation provides evidence that it is the transfer of small molecules, nucleotide, or nucleotide derivatives that is responsible for correction of the mutant phenotype. Karyoplasts (nuclei with small amounts of cytoplasm surrounded by a plasma membrane) are unable to efficiently communicate with intact cells. The utilization of [3H]hypoxanthine by communicating mixtures of HPRT+ and HPRT− human cells is not significantly different than in the normal cells alone. Metabolic cooperation, as studied, involves a redistribution of purine-containing compounds among communicating cells.
AB - Metabolic cooperation, the correction of the mutant phenotype in cells deficient in hypoxanthine phosphoribosyltransferase (HPRT−) by intimate contact with normal cells (HPRT+), represents a form of cell commimication that is easily studied with radioautography. In the present study it was found that the formation of cell junctions needed for communication does not require protein synthesis nor is it under the immediate control of the cell nucleus. Enucleated normal cells efficiently communicate with HPRT− mutant cells. The effectiveness of enucleated cells as donors in metabolic cooperation provides evidence that it is the transfer of small molecules, nucleotide, or nucleotide derivatives that is responsible for correction of the mutant phenotype. Karyoplasts (nuclei with small amounts of cytoplasm surrounded by a plasma membrane) are unable to efficiently communicate with intact cells. The utilization of [3H]hypoxanthine by communicating mixtures of HPRT+ and HPRT− human cells is not significantly different than in the normal cells alone. Metabolic cooperation, as studied, involves a redistribution of purine-containing compounds among communicating cells.
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U2 - 10.1083/jcb.71.3.693
DO - 10.1083/jcb.71.3.693
M3 - Article
C2 - 993266
AN - SCOPUS:0017150007
SN - 0021-9525
VL - 71
SP - 693
EP - 703
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 3
ER -