Studies on cell communication with enucleated human fibroblasts

R. P. Cox, M. R. Krauss, M. E. Balis, J. Dancis

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Metabolic cooperation, the correction of the mutant phenotype in cells deficient in hypoxanthine phosphoribosyltransferase (HPRT-) by intimate contact with normal cells (HPRT+), represents a form of cell communication that is easily studied with radioautography. In the present study it was found that the formation of cell junctions needed for communication does not require protein synthesis nor is it under the immediate control of the cell nucleus. Enucleated normal cells efficiently communicate with HPRT- mutant cells. The effectiveness of enucleated cells as donors in metabolic cooperation provides evidence that it is the transfer of small molecules, nucleotide, or nucleotide derivatives that is responsible for correction of the mutant phenotype. Karyoplasts (nuclei with small amounts of cytoplasm surrounded by a plasma membrane) are unable to efficiently communicate with intact cells. The utilization of [3H]hypoxanthine by communicating mixtures of HPRT+ and HPRT- human cells is not significantly different than in the normal cells alone. Metabolic cooperation, as studied, involves a redistribution of purine containing compounds among communicating cells.

Original languageEnglish (US)
Pages (from-to)693-703
Number of pages11
JournalJournal of Cell Biology
Volume71
Issue number3
StatePublished - 1976

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Cell Communication
Fibroblasts
Hypoxanthine Phosphoribosyltransferase
Nucleotides
Phenotype
Hypoxanthine
Intercellular Junctions
Autoradiography
Cell Nucleus
Cytoplasm
Communication
Cell Membrane

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cox, R. P., Krauss, M. R., Balis, M. E., & Dancis, J. (1976). Studies on cell communication with enucleated human fibroblasts. Journal of Cell Biology, 71(3), 693-703.

Studies on cell communication with enucleated human fibroblasts. / Cox, R. P.; Krauss, M. R.; Balis, M. E.; Dancis, J.

In: Journal of Cell Biology, Vol. 71, No. 3, 1976, p. 693-703.

Research output: Contribution to journalArticle

Cox, RP, Krauss, MR, Balis, ME & Dancis, J 1976, 'Studies on cell communication with enucleated human fibroblasts', Journal of Cell Biology, vol. 71, no. 3, pp. 693-703.
Cox, R. P. ; Krauss, M. R. ; Balis, M. E. ; Dancis, J. / Studies on cell communication with enucleated human fibroblasts. In: Journal of Cell Biology. 1976 ; Vol. 71, No. 3. pp. 693-703.
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