TY - JOUR
T1 - Studies on the mechanism of adsorption of purines in Sephadex G-10 chromatography
AU - Sweetman, Lawrence
AU - Nyhan, William L.
N1 - Funding Information:
Work clone in part in Departments of Pediatrics and I~iocliemistry, School of Medicine, University of Miami, Miami, Fla. 33152, U.S.A. Supported in part by National Institutes of Health Grant CA-0574s and USPHS Research Grant No. HD 0460s from the National Institute of Child Health ancl I-Iuman Development, NIH.
PY - 1971
Y1 - 1971
N2 - The adsorption of purines to Sephadex G-10, as measured by the logarithms of corrected chromatographic elution volumes, Ve0, shows correlations with the following physical properties of the purines: π-electron delocalization energy, electron donating ability, electron accepting ability, basic and acidic pKa and water solubility. These correlations, together with equilibrium dialysis studies of adsorption of purines to dextran and Sephadex, indicate that purines interact primarily with the dextran portion of the Sephadex in contrast to substituted benzene derivatives, which interact primarily with the ether cross-linkages of Sephadex.
AB - The adsorption of purines to Sephadex G-10, as measured by the logarithms of corrected chromatographic elution volumes, Ve0, shows correlations with the following physical properties of the purines: π-electron delocalization energy, electron donating ability, electron accepting ability, basic and acidic pKa and water solubility. These correlations, together with equilibrium dialysis studies of adsorption of purines to dextran and Sephadex, indicate that purines interact primarily with the dextran portion of the Sephadex in contrast to substituted benzene derivatives, which interact primarily with the ether cross-linkages of Sephadex.
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U2 - 10.1016/S0021-9673(01)82227-5
DO - 10.1016/S0021-9673(01)82227-5
M3 - Article
C2 - 5110718
AN - SCOPUS:0015236476
SN - 0021-9673
VL - 59
SP - 349
EP - 366
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 2
ER -