Studies on the mechanism of adsorption of purines in Sephadex G-10 chromatography

Lawrence Sweetman, William L. Nyhan

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

The adsorption of purines to Sephadex G-10, as measured by the logarithms of corrected chromatographic elution volumes, Ve0, shows correlations with the following physical properties of the purines: π-electron delocalization energy, electron donating ability, electron accepting ability, basic and acidic pKa and water solubility. These correlations, together with equilibrium dialysis studies of adsorption of purines to dextran and Sephadex, indicate that purines interact primarily with the dextran portion of the Sephadex in contrast to substituted benzene derivatives, which interact primarily with the ether cross-linkages of Sephadex.

Original languageEnglish (US)
Pages (from-to)349-366
Number of pages18
JournalJournal of Chromatography A
Volume59
Issue number2
DOIs
StatePublished - 1971
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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