Subcellular localization of immunoreactive α-melanocyte stimulating hormone in human brain

C. Richard Parker, John C. Porter

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

The regional and subcellular distribution of immunoreactive α-melanocyte stimulating hormone (α-MSH1) in the post mortem adult human brain was investigated. α-MSHi was highly concentrated in medial basal hypothalamic tissue (1.02 ng/mg protein). Lower levels of α-MSHi were present in the optic chiasm and mammillary bodies, 0.08 and 0.11 ng/mg protein, respectively. The concentrations of α-MSHi in cerebellum and frontal cerebral cortex were 1/1,000th that of the medial basal hypothalamus. When medial basal hypothalamic homogenates were subjected to discontinuous or continuous sucrose density gradients, α-MSHi was found to be associated primarily with subcellular particles which resembled isolated nerve terminals, i.e., synaptosomes. Low to undetectable amounts of α-MSHi were found in the cytosol or the myelin/microsome fraction of the gradients. The results of these studies are consistent with the view that α-MSH is a neuronal peptide in the human brain.

Original languageEnglish (US)
Pages (from-to)535-538
Number of pages4
JournalBrain Research Bulletin
Volume4
Issue number4
DOIs
StatePublished - Jan 1 1979

Keywords

  • Human brain
  • Hypothalamus
  • Neuronal peptide
  • Subcellular fractionation
  • Synaptosomes
  • α-MSH
  • α-Melanocyte stimulating hormone

ASJC Scopus subject areas

  • Neuroscience(all)

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